cDNA Cloning and Functional Analyses of Ashitaba (Angelica keiskei) Sesquiterpene Synthase Genes

Angelica keiskei (ashitaba) is an edible plant belonging to the Apiacea household. We centered on sesquiterpenes within the leaves eaten by people (particularly, within the Japanese inhabitants), and confirmed the presence of a number of sesquiterpenes by GC-MS. Thus, whole RNA was extracted from the ashitaba leaves, reverse transcribed, and the resultant cDNAs have been used for degenerate PCR adopted by speedy amplification of cDNA ends. Consequently, we have been in a position to isolate two full-length Tps genes (designated AkTps1 and AkTps2).

Purposeful evaluation of those two genes was carried out with Escherichia coli cells that expressed mevalonate pathway genes to extend the substrate (farnesyl diphosphate) quantity of sesquiterpene synthase, revealing that AkTps1 encodes germacrene D synthase, and AkTps2 codes for an enzyme that catalyzes the technology of germacrene B and smaller quantities of germacrene D (a germacrene B and D synthase). We proposed biosynthetic routes of those two sesquiterpenes from farnesyl diphosphate (FPP) through farnesyl cation.

Stabilization of a full-length infectious cDNA clone for duck Tembusu virus by insertion of an intron

Duck Tembusu virus (DTMUV) belongs to the genus Flavivirus, household Flaviviridae. In our beforehand examine, a full-length cDNA clone of DTMUV was constructed, nevertheless, it’s vulnerable to mutation throughout genetic engineering as a result of prokaryotic toxicity of viral protein, which can also be a typical characteristic for flavivirus.

On this examine, we reported an intron-containing full-length cDNA clone for a medical pressure CQW1, the intro (133bp) was inserted into nonstructural protein 1 of DTMUV at 192 web site. This intron-containing full-length cDNA clone was stably propagated in Escherichia coli with out prokaryotic toxicity, and recombinant virus was produced by direct transfection of plasmids. In addition to, this cDNA clone-derived recombinant virus confirmed comparable properties as compared with guardian virus each in vitro and in vivo. It is handy and environment friendly, making it a helpful platform for the following analysis of reverse genetics of flavivirus.

 

Identification of the cDNA Encoding the Development Hormone Receptor ( GHR) and the Regulation of GHR and IGF-I Gene Expression by Dietary Standing in Reeves’ Turtle ( Chinemys reevesii)

Chinemys reevesii (Reeves’ turtle) is a slow-growing reptile that’s distributed broadly throughout China. Previous to this examine, the cDNA sequence of the expansion hormone receptor (GHR) within the Reeve’s turtle, or how intervals of hunger would possibly affect the gene expression of GHR and insulin-like development issue I (IGF-I) on this species, have been unknown. Right here, we recognized the full-length sequence of the cDNA encoding GHR in Reeves’ turtle through the use of RT-PCR and RACE. The total-length GHR cDNA was recognized to be 3936 base-pairs in size, with a 1848 base-pair open studying body (ORF) that encodes a 615 amino acid protein.

Evaluation confirmed that GHR mRNA was detectable in a variety of tissues; the best and lowest ranges of expression have been detected within the liver and the gonad, respectively. IGF-I was additionally expressed in a spread of tissues, however not within the gonad; the best ranges of IGF-I expression have been detected within the liver. After four weeks of fasting, the expression ranges of GHR and IGF-I within the liver had decreased considerably; nevertheless, these step by step returned to regular after refeeding.

We report the primary cloned cDNA sequence for the GHR gene within the Reeve’s turtle. Our findings present a basis from which to research the precise perform of the GHR in Reeve’s turtle, and function a reference for learning the results of various nutrient ranges on GHR expression on this species.

Building of secure infectious full-length and eGFP-tagged cDNA clones of Mirabilis crinkle mosaic virus through In-Fusion cloning

Mirabilis crinkle mosaic virus (MiCMV) was tentatively categorized as a brand new member of the genus Potyvirus within the household Potyviridae in 2019. Nonetheless, it was thought of to be basella rugose mosaic virus primarily based on the sequence similarity of the coat protein (CP) area. On this examine, infectious MiCMV cDNA clones beneath the management of the 35S promoter have been constructed with an In-Fusion cloning technique.

Systemically contaminated leaves of Mirabilis jalapa and Nicotiana benthamiana crops inoculated with pMiCMV and pMiCMV-NIb/eGFP had mosaic signs by 5 dpi. Infections have been confirmed by a western blot evaluation, electron microscopy, RT-PCR, and the inoculation of N. benthamiana seedlings with progeny virions.

Fetus (17 Day Fetus) Lysate
21-392	ProSci	0.1 mg	EUR 342.6
Description: Mouse fetus (17 day fetus) tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The mouse fetus (17 day fetus) tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the fetus (17 day fetus) tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The fetus (17 day fetus) tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Fetus (11 Day Fetus) Lysate
21-467	ProSci	0.1 mg	EUR 342.6
Description: Rat fetus (11 day fetus) tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The rat fetus (11 day fetus) tissue total protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the fetus (11 day fetus) tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The fetus (11 day fetus) tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Lactation Elevated Protein 1 (LACE1) Antibody
abx025801-400ul	Abbexa	400 ul	EUR 627.6

Lactation Elevated Protein 1 (LACE1) Antibody
abx025801-80l	Abbexa	80 µl	EUR 343.2

Lactation Elevated Protein 1 (LACE1) Antibody
abx036538-100ug	Abbexa	100 ug	EUR 469.2

Lactation Elevated Protein 1 (LACE1) Antibody
20-abx318622	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Chicken (Normal/Adult) Universal (pool of tissue cDNA)
CHUC-565	Alpha Diagnostics	10 rxn	EUR 498

Extension of On-going custom antibody Order form
Ext-3	Alpha Diagnostics	1	Ask for price

Heart Lysate (0 day old mouse)
1401-0	ProSci	0.1 mg	EUR 229.2
Description: Heart tissue lysate (0 day old mouse) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Heart Lysate (7 day old mouse)
1401-7	ProSci	0.1 mg	EUR 229.2
Description: Heart tissue lysate (7 day old mouse) was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Tristel One Day Concentrate For Labs
TRI1394	Scientific Laboratory Supplies	PK2	EUR 241.68

Lactation Elevated Protein 1 (LACE1) Antibody (HRP)
20-abx308834	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Lactation Elevated Protein 1 (LACE1) Antibody (FITC)
20-abx308835	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

Lactation Elevated Protein 1 (LACE1) Antibody (Biotin)
20-abx308836	Abbexa	EUR 493.20 EUR 2214.00 EUR 718.80 EUR 218.40 EUR 360.00	100 ug 1 mg 200 ug 20 ug 50 ug

CrystalDirect Crystallization Plates; Pack of 3
M-XDIR-96-2-3	MiTeGen	3 PLATES	EUR 64
Description: CrystalDirect Crystallization Plates; Pack of 3

Recombinant Transmembrane Protease Serine 2 Protein, Partial
E80016	EpiGentek	EUR 470.80 EUR 753.50 EUR 1824.90	20 ul 100 ul 500 ul

Recombinant TMPRSS2 Protein, Partial
E80017	EpiGentek	EUR 423.50 EUR 660.00 EUR 2321.00	20 ul 100 ul 1 ml

Recombinant Novel Coronavirus Spike Glycoprotein(S), Partial
E80018	EpiGentek	EUR 388.30 EUR 860.20 EUR 4888.40	20 ul 100 ul 1 ml

MicroCrystal Mount Assortment, box of 20; Model #3, on 18mm rods
M4-L18SP-3	MiTeGen	20 MOUNTS	EUR 125
Description: MicroCrystal Mount Assortment, box of 20; Model #3, on 18mm rods

Lethal factor Protease Substrate 3, AMC derivative MEk2 peptide substrate for high-throughput screening of LF inhibitors (14aa)
LFPS-3	Alpha Diagnostics	1 mg	EUR 489.6

TIMP2 Human, Tissue Inhibitor of Metalloprotease 2 Human Recombinant Protein, Sf9
PROTP16035-3	BosterBio	Regular: 5ug	EUR 380.4
Description: TIMP2 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 200 amino acids (27-220a.a.) and having a molecular mass of 22.5kDa. (Molecular size on SDS-PAGE will appear at approximately 18-28kDa). TIMP2 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Human Lactation Elevated Protein 1 (AFG1L) ELISA Kit
abx385075-96tests	Abbexa	96 tests	EUR 1093.2

Rat Lactation elevated protein 1 (LACE1) ELISA Kit
abx391534-96tests	Abbexa	96 tests	EUR 1093.2

Mouse Lactation elevated protein 2 (LACE1) ELISA Kit
abx389701-96tests	Abbexa	96 tests	EUR 1093.2

Human Lactation elevated protein 1, LACE1 ELISA KIT
ELI-42677h	Lifescience Market	96 Tests	EUR 988.8

Mouse Lactation elevated protein 1, Lace1 ELISA KIT
ELI-28039m	Lifescience Market	96 Tests	EUR 1038

Cube Ice Machine 150kg/day storage 75kg
LITV-DP150	Scientific Laboratory Supplies	EACH	EUR 6441

Cube Ice Machine 35kg/day storage 20kg
LITV-DP35H	Scientific Laboratory Supplies	EACH	EUR 2912.7

Cube Ice Machine 81kg/day storage 35kg
LITV-DP80	Scientific Laboratory Supplies	EACH	EUR 4306.92

Flake Ice Machine 156kg/day storage 40kg
LITV-IQ135	Scientific Laboratory Supplies	EACH	EUR 6441

Flake Ice Machine 234kg/day storage 80kg
LITV-IQ200	Scientific Laboratory Supplies	EACH	EUR 8228.52

Flake Ice Machine 66kg/day storage 20kg
LITV-IQ50	Scientific Laboratory Supplies	EACH	EUR 4332

Flake Ice Machine 90kg/day storage 20kg
LITV-IQ85C	Scientific Laboratory Supplies	EACH	EUR 5232.6

Cube Ice Machine 20kg/day storage 6kg
LITV-NDP20	Scientific Laboratory Supplies	EACH	EUR 2188.8

Livingcare Group Covid-19 Day 2 Testing
COV3000	Scientific Laboratory Supplies	PK200	EUR 2763.36

Paraffin Tissue Section - Human Lymph Nodes Tumor: Reactive Hyperplasia of Lymph Node
T2235161-3	Biochain	5 slides	EUR 308.4
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

cDNA Synthesis SuperMix
20-abx09801420ulSystems	Abbexa	EUR 678.00 EUR 577.20	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

Novo? cDNA Kit
M1165-100	Biovision	each	EUR 424.8

Novo? cDNA Kit
M1165-25	Biovision	each	EUR 320.4

Evo? cDNA Supermix
M1168-100	Biovision	each	EUR 457.2

Evo? cDNA Supermix
M1168-25	Biovision	each	EUR 320.4

Novo? cDNA Supermix
M1169-100	Biovision	each	EUR 529.2

Novo? cDNA Supermix
M1169-25	Biovision	each	EUR 346.8

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis
C1634310	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn
C1634330	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange
C1634340	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato
C1634350	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice
C1634360	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat
C1634390	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

EpiQuik Dnmt1 Assay Kit
P-3011	EpiGentek	EUR 1067.46 EUR 512.60 EUR 724.90	96 Assays 48 Assays 96 Assays

EpiQuik Dnmt3A Assay Kit
P-3012	EpiGentek	EUR 1067.46 EUR 503.80 EUR 710.60	96 Assays 48 Assays 96 Assays

EpiQuik Dnmt3B Assay Kit
P-3013	EpiGentek	EUR 1067.46 EUR 503.80 EUR 710.60	96 Assays 48 Assays 96 Assays

3-aminobenzamide
79888-3	BPS Bioscience	5 g	EUR 250
Description: 3-aminobenzamide is a novel, potent inhibitor of PARP. Also acts as a mediator of oxidant-induced myocyte dysfunction during reperfusion.

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)
20-abx09801620ulSystems	Abbexa	EUR 744.00 EUR 627.60	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)
20-abx09802120ulSystems	Abbexa	EUR 1045.20 EUR 794.40	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean
C1634370	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Lace1 ELISA Kit| Mouse Lactation elevated protein 1 ELISA Kit
EF015336	Lifescience Market	96 Tests	EUR 826.8

Lace1 ELISA Kit| Rat Lactation elevated protein 1 ELISA Kit
EF018892	Lifescience Market	96 Tests	EUR 826.8

Tetro cDNA Synthesis Kit
BIO-65042	Bioline	30 Reactions	Ask for price

Tetro cDNA Synthesis Kit
BIO-65043	Bioline	100 Reactions	Ask for price

SensiFAST cDNA Synthesis Kit
BIO-65053	Bioline	50 Reactions	Ask for price

SensiFAST cDNA Synthesis Kit
BIO-65053/S	Bioline	Sample	Ask for price

SensiFAST cDNA Synthesis Kit
BIO-65054	Bioline	250 Reactions	Ask for price

cDNA Probe Diluent Solution
AR0063	BosterBio	5mL	EUR 127.2

cDNA from Arteriosclerosis: Aorta
C1236012Hd-4	Biochain	40 reactions	EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Artery
C1236013Hd-2	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Arteriosclerosis: Artery
C1236013Hd-4	Biochain	40 reactions	EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Vein
C1236020Hd-2	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Colon
C1236090Lup	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Heart
C1236122Hd-2	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Heart
C1236122Lup	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Kidney
C1236142Hd-2	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Kidney
C1236142Lup	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Liver
C1236149Lup	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Asthma: Lung
C1236152Ld-1	Biochain	40 reactions	EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Bronchitis: Lung
C1236152Ld-2	Biochain	40 reactions	EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pneumonia: Lung
C1236152Ld-4	Biochain	40 reactions	EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Lung
C1236152Lup	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Pancreas
C1236188Lup	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spleen
C1236246Lup	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: stomach
C1236248Lup	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human eNOS cDNA probe
eNOS51-D-2	Alpha Diagnostics	2 ug	EUR 534

OneScriptPlus cDNA Synthesis Kit
G235	ABM	25 x 20 ul reactions	EUR 116.4

OneScriptPlus cDNA Synthesis Kit
G236	ABM	100 x 20 ul reactions	EUR 202.8

OneScriptPlus cDNA Synthesis SuperMix
G453	ABM	25 x 20 ul reactions	EUR 116.4

OneScriptPlus cDNA Synthesis SuperMix
G454	ABM	100 x 20 ul reactions	EUR 202.8

circRNA cDNA Synthesis Kit
G627	ABM	25 rxn (20 ul/rxn)	EUR 370.8

Plant Tissue cDNA: Arabidopsis
PC34-310	Alpha Diagnostics	10 rxn	EUR 498

Evo™ cDNA Kit
M1164-100	Biovision	each	Ask for price

Evo™ cDNA Kit
M1164-25	Biovision	each	Ask for price

Novo? Transcriptome cDNA Kit
M1167-100	Biovision	each	EUR 1142.4

Novo? Transcriptome cDNA Kit
M1167-25	Biovision	each	EUR 529.2

EVery cDNA Synthesis Kit
EVery200B-1	SBI	20 preps	EUR 307

Anti-14-3-3 alpha + beta Rabbit Monoclonal Antibody
M02431-3	BosterBio	100ug/vial	EUR 476.4
Description: Rabbit Monoclonal 14-3-3 alpha + beta Antibody. Validated in Flow Cytometry, IP, IF, IHC, ICC, WB and tested in Human, Mouse, Rat.

IL-3 Interleukin-3 Human Recombinant Protein, His Tag
PROTP08700-3	BosterBio	Regular: 50ug	EUR 380.4
Description: Interleukin-3 Human Recombinant produced in E.Coli is single, a non-glycosylated, Polypeptide chain containing 154 amino acids fragment (20-152) and having a total molecular mass of 17.3kDa and fused with a 20 aa N-terminal His tag. ;The IL3 His is purified by proprietary chromatographic techniques.

Individual Reaction Mix 3
G065-3	ABM	200 reactions	EUR 200.4

3-D Life Thioglycerol
T10-3	Cellendes	180 µl	EUR 57.6

Organic Wine Standard 3
WINE-3	Scientific Laboratory Supplies	1ML	EUR 258.78

Western Blot Box - 2 7/8 x 1 3/16 x 3/4in.; 7.3 x 3 x 1.9cm
B1200-3	MTC Bio	5/pack	EUR 76.52
Description: Western Blot Boxes

TGF-b-3 Transforming Growth Factor-Beta 3 Human Recombinant Protein, Plant
PROTP10600-3	BosterBio	Regular: 5ug	EUR 380.4
Description: TGFB3 Human Recombinant produced in plant is a disulfide-linked homodimeric, glycosylated, polypeptide chain containing 118 amino acids and having a molecular mass of 27.2kDa. ;The TGFB3 is fused to 6xHis tag at N-terminus and purified by standard chromatographic techniques.

3-D-Life SG-PVA
M81-3	Cellendes	3x 170 µl	EUR 177.6

3-D Life FG-PVA
M82-3	Cellendes	3x 170 µl	EUR 177.6

3-D Life SG-Dextran
M91-3	Cellendes	3x 170 µl	EUR 188.4

3-D Life FG-Dextran
M92-3	Cellendes	3x 170 µl	EUR 188.4

3-D Life RGD Peptide
P10-3	Cellendes	3x 1 µmol	EUR 331.2

3-D Life PEG-Link
L50-3	Cellendes	3x 200 µl	EUR 112.8

3-D Life CD-Link
L60-3	Cellendes	3 x 200 µl	EUR 385.2

Dr. P Kit-Solution 3
K2021010-3	Biochain	50 ml	EUR 159.6
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Recombinant Human MMP-3 Protein
PROTP08254-3	BosterBio	10ug	EUR 380.4
Description: Matrix metalloproteinases (MMPs) are a family of endoproteases that require zinc and calcium for expressing catalytic activity. These enzymes play a central role in the maintenance and remodeling of the extracellular matrix. Elevated expression of their activity, caused either by up-regulation of their expression or down-regulation of their cognate inhibitors, has been implicated in various degenerative disorders, including arthritis, cardiovascular disease, skeletal growth-plate disorders, and cancer metastasis. MMP-3 degrades fibronectin, laminin, collagens III, IV, and X, and cartilage proteoglycans. Recombinant human MMP-3 is a 42.8 kDa protein containing the entire catalytic N-terminal domain and the C-terminal domain (378 amino acids).

pLenti-CARM1 shRNA-3 Plasmid
PVTBAV03691-3	Lifescience Market	2 ug	EUR 427.2

pLenti-CTLA4 shRNA-3 Plasmid
PVTBAV05689-3	Lifescience Market	2 ug	EUR 427.2

pLenti-FOXM1 shRNA-3 Plasmid
PVTBAV08732-3	Lifescience Market	2 ug	EUR 427.2

pLenti-JUN shRNA-3 Plasmid
PVTBAV11741-3	Lifescience Market	2 ug	EUR 427.2

pLenti-LHX6 shRNA-3 Plasmid
PVTBAV12881-3	Lifescience Market	2 ug	EUR 427.2

pLenti-MAGEA3 shRNA-3 Plasmid
PVTBAV13661-3	Lifescience Market	2 ug	EUR 427.2

pLenti-RUNX3 shRNA-3 Plasmid
PVTBAV20583-3	Lifescience Market	2 ug	EUR 427.2

pLenti-Slc7a11 shRNA-3 Plasmid
PVTBAV21973-3	Lifescience Market	2 ug	EUR 427.2

pLenti-STAT3 shRNA-3 Plasmid
PVTBAV22921-3	Lifescience Market	2 ug	EUR 427.2

Caspase-3/7 Inhibitor I
27741-3	BPS Bioscience	25 mg	EUR 700
Description: A potent, cell-permeable, and specific, reversible inhibitor of caspase-3 and caspase-7.

FSH (Human Follicle-stimulating hormone) ELISA test
3	Biobase	96T/Box	Ask for price
Description: ELISA based test for quantitative detection of FSH (Human Follicle-stimulating hormone)

Anti-VEGF Receptor 3/FLT4 Antibody
A01276-3	BosterBio	100ug/vial	EUR 400.8

3-D Life Scrambled RGD Peptide
P11-3	Cellendes	3x 1 µmol	EUR 331.2

ICP-MS Multi-Element Solution 3
CLMS-3	Scientific Laboratory Supplies	125ML	EUR 524.4

Florentine Flask Fd 100/3 100ml
QFD100/3	Scientific Laboratory Supplies	EACH	EUR 35.56

Caspase-3 Inhibitor Q-DEVD-OPh
1175-3	Biovision	each	EUR 639.6

Anti-active Caspase-3 Rabbit Monoclonal Antibody
M00334-3	BosterBio	100ug/vial	EUR 476.4
Description: Anti-active Caspase-3 Rabbit Monoclonal Antibody tested for IF, IHC, ICC, WB in Human

Anti-Galectin 3/LGALS3 Antibody (monoclonal, 12B12)
M00621-3	BosterBio	100ug/vial	EUR 400.8

QuantiMir 3'-Universal Primer (10,000 qPCR reactions)
RA420AU-3	SBI	10,000 assays	EUR 213.6

SeraMir 3'-Universal Primer (10,000 qPCR reactions)
RA800P-3	SBI	10,000 reactions	EUR 213.6

Drying Tube Angled Mf23/3 24/29
QMF23/3	Scientific Laboratory Supplies	EACH	EUR 41.87

Rabbit Complement 3-4 WK (100-mL)
31061-3	Pel-Freez	100mL	EUR 1322.98

cDNA Synthesis SuperMix for qPCR
20-abx09801920ulSystems	Abbexa	EUR 828.00 EUR 678.00	100 rxns ×20 ul Systems 50 rxns × 20 ul Systems

cDNA from Alzheimer's Disease: Brain
C1236035Alz	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Brain
C1236035Lcs	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Parkinson's Disease: Brain
C1236035Par	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dementia: Brain: Hippocampus
C1236052Dem	Biochain	40 reactions	EUR 961.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Depression: Brain: Hippocampus
C1236052Dep	Biochain	40 reactions	EUR 961.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Colon
C1236090Lcs	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Esophagus
C1236106Lcs	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Heart
C1236122Lcs	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Interventricular Septum
C1236130Hd-2	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Kidney
C1236142Lcs	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Liver
C1236149Lcs	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Lung
C1236152Lcs	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Systemic infections weren’t noticed after Nicotiana glutinosa leaves have been equally inoculated, with eGFP fluorescence detected solely within the inoculated leaves. Apparently, the signs induced by pMiCMV and pMiCMV-NIb/eGFP weren’t just like these attributable to the wild-type MiCMV in Basella rubra crops. Furthermore, RT-PCR analyses of B. rubra crops with virus-specific primers (MicpF and MicpR) indicated {that a} non-target fragment akin to the MiCMV CP coding area was amplified. That is the primary report of the development of a biologically energetic, full-length cDNA copy of the MiCMV RNA genome.