The London airplane tree (Platanus acerifolia Willd.) has world significance as an city landscaping tree and is the topic of genetic-improvement applications for productive sterility, illness and/or insect resistance. Molecular evaluation methods are essential to such applications, however could also be impeded by particular difficulties encountered throughout nucleic acid isolation.
An in depth RNA isolation and purification protocol, based mostly on established cetyltrimethyl-ammonium bromide (CTAB) extraction methods mixed with further purification steps utilizing butanol and the ionic detergent CTAB, which overcomes these issues within the woody species P. acerifolia, was performed. In brief, phenolic compounds are certain to soluble polyvinylpyrrolidone after which separated out via LiCl precipitation of the RNA. Subsequently, protein- and carbohydrate-contaminants are eliminated by chloroform partitioning adopted by LiCl-mediated precipitation.
The ensuing isolates of RNA have been discovered to be of enough high quality for profitable use in reverse transcription PCR evaluation. Moreover, RNA isolates from feminine inflorescences have been used for the development of a cDNA library. This library was discovered to comprise a number of full-length cDNA clones of MADS-box genes, according to the library being consultant of inflorescence expression profiles.
Molecular evaluation of a stress-induced cDNA encoding the interpretation initiation issue, eIF1, from the salt-tolerant wild relative of rice, Porteresia coarctata
The evaluation of plant response to emphasize is a crucial path to the invention of genes conferring stress tolerance. Protein synthesis may be very delicate to salt stress and proteins concerned on this course of could also be an necessary determinant of salt tolerance. The halophytic plant, Porteresia coarctata Tateoka, is an in depth relative of Oryza sativa L., and has the power to face up to sudden adjustments within the soil salinity.
The interpretation initiation issue 1 (PceIF1) cDNA was remoted from the leaves of P. coarctata that had been subjected to a high-salt remedy (150 mm NaCl). An expression research confirmed that the abundance of eIF1 transcripts elevated to a most degree 5 d after stress induction after which decreased to ranges much like leaves of management (unsalinised) crops.
This gene was additionally up-regulated in exogenous abscisic acid (ABA) and mannitol remedies, suggesting that its induction is expounded to the water deficit impact of excessive salt. Our research confirmed that expression ranges of eIF1 transcripts may kind a handy indicator for monitoring a stress-responsive mechanism that operates within the leaves of P. coarctata.
Transcriptome evaluation of leaf tissue from Bermudagrass (Cynodon dactylon) utilizing a normalised cDNA library
A normalised cDNA library was constructed from Bermudagrass to achieve perception into the transcriptome of Cynodon dactylon L. A complete of 15 588 high-quality expressed sequence tags (ESTs) from the cDNA library have been subjected to The Institute for Genomic Analysis Gene Indices clustering instruments to supply a unigene set. A complete of 9414 unigenes have been obtained from the high-quality ESTs and solely 39.6% of the high-quality ESTs have been redundant, indicating that the normalisation process was efficient.
A big-scale comparative genomic evaluation of the unigenes was carried out utilizing publicly out there instruments, resembling BLAST, InterProScan and Gene Ontology. The unigenes have been additionally subjected to a seek for EST-derived easy sequence repeats (EST-SSRs) and conserved-intron scanning primers (CISPs), that are helpful as DNA markers.

Though the candidate EST-SSRs and CISPs discovered within the current research have to be empirically examined, they’re anticipated to be helpful as DNA markers for a lot of functions, together with comparative genomic research of grass species, by advantage of their vital similarities to EST sequences from different grasses. Thus, information of Cynodon ESTs will empower turfgrass analysis by offering homologues for genes which can be thought to confer necessary capabilities in different crops.
Lengthy-read cDNA Sequencing Permits a ‘Gene-Like’ Transcript Annotation of Transposable Parts
Transcript-based annotations of genes facilitate each genome-wide analyses and detailed single locus analysis. In distinction, transposable factor (TE) annotations are rudimentary, consisting of knowledge solely on TE location and sort. The repetitiveness and restricted annotation of TEs prevents the power to differentiate between probably practical expressed parts and degraded copies.
To enhance genome-wide TE bioinformatics, we carried out long-read sequencing of cDNAs from Arabidopsis thaliana traces poor in a number of layers of TE repression. These uniquely-mapping transcripts have been used to determine the set of TEs in a position to generate polyadenylated RNAs and create a brand new transcript-based annotation of TEs that we’ve layered upon the prevailing high-quality group commonplace annotation.
cDNA from Monkey (Cynomolgus) Normal Tissue: Uterus: Cervix |
C1534275-Cy |
Biochain |
40 reactions |
EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Monkey (Rhesus) Normal Tissue: Uterus: Corpus |
C1534276 |
Biochain |
40 reactions |
EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Monkey (Rhesus) Normal Tissue: Uterus: Fundus |
C1534278 |
Biochain |
40 reactions |
EUR 540 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Human Catalase (CAT) ELISA Kit |
DLR-CAT-Hu-48T |
DL Develop |
48T |
EUR 441 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Human Catalase (CAT) ELISA Kit |
DLR-CAT-Hu-96T |
DL Develop |
96T |
EUR 570 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Human Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Mouse Catalase (CAT) ELISA Kit |
DLR-CAT-Mu-48T |
DL Develop |
48T |
EUR 527 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Mouse Catalase (CAT) ELISA Kit |
DLR-CAT-Mu-96T |
DL Develop |
96T |
EUR 688 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Rat Catalase (CAT) ELISA Kit |
DLR-CAT-Ra-48T |
DL Develop |
48T |
EUR 549 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Rat Catalase (CAT) ELISA Kit |
DLR-CAT-Ra-96T |
DL Develop |
96T |
EUR 718 |
|
Description: A sandwich quantitative ELISA assay kit for detection of Rat Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids. |
Human Catalase (CAT) ELISA Kit |
RD-CAT-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 436 |
Human Catalase (CAT) ELISA Kit |
RD-CAT-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 601 |
Mouse Catalase (CAT) ELISA Kit |
RD-CAT-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 533 |
Mouse Catalase (CAT) ELISA Kit |
RD-CAT-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 740 |
Rat Catalase (CAT) ELISA Kit |
RD-CAT-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 557 |
Rat Catalase (CAT) ELISA Kit |
RD-CAT-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 775 |
Human Catalase (CAT) ELISA Kit |
RDR-CAT-Hu-48Tests |
Reddot Biotech |
48 Tests |
EUR 455 |
Human Catalase (CAT) ELISA Kit |
RDR-CAT-Hu-96Tests |
Reddot Biotech |
96 Tests |
EUR 629 |
Mouse Catalase (CAT) ELISA Kit |
RDR-CAT-Mu-48Tests |
Reddot Biotech |
48 Tests |
EUR 557 |
Mouse Catalase (CAT) ELISA Kit |
RDR-CAT-Mu-96Tests |
Reddot Biotech |
96 Tests |
EUR 774 |
Rat Catalase (CAT) ELISA Kit |
RDR-CAT-Ra-48Tests |
Reddot Biotech |
48 Tests |
EUR 583 |
Rat Catalase (CAT) ELISA Kit |
RDR-CAT-Ra-96Tests |
Reddot Biotech |
96 Tests |
EUR 811 |
Uterus Lysate |
1317 |
ProSci |
0.1 mg |
EUR 191 |
Description: Uterus tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Uterus Tumor Lysate |
1384 |
ProSci |
0.1 mg |
EUR 254 |
Description: Uterus tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT. |
Fetal Uterus Lysate |
XBL-10431 |
ProSci |
0.1 mg |
EUR 527 |
Description: Fetal human uterus tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human uterus tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the uterus tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The uterus tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot. |
Uterus Membrane Lysate |
XBL-11023 |
ProSci |
0.1 mg |
EUR 516.5 |
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
Bovine UTERUS 5 EA* |
57153-2 |
Pel-Freez |
5 EA |
EUR 204.2 |
Uterus Tissue Slide (Normal) |
11-401-10um |
ProSci |
10 um |
EUR 201.5 |
Uterus Tissue Slide (Normal) |
11-401-4um |
ProSci |
4 um |
EUR 180.5 |
Uterus Tissue Slide (Benign) |
11-402-10um |
ProSci |
10 um |
EUR 201.5 |
Uterus Tissue Slide (Benign) |
11-402-4um |
ProSci |
4 um |
EUR 180.5 |
Uterus Tissue Slide (Adenocarcinoma) |
11-404-10um |
ProSci |
10 um |
EUR 201.5 |
Uterus Tissue Slide (Adenocarcinoma) |
11-404-4um |
ProSci |
4 um |
EUR 180.5 |
Uterus Tissue Slide (Abnormal) |
11-419-10um |
ProSci |
10 um |
EUR 201.5 |
Uterus Tissue Slide (Abnormal) |
11-419-4um |
ProSci |
4 um |
EUR 180.5 |
Uterus Tissue Slide (Adenomyoma) |
11-432-10um |
ProSci |
10 um |
EUR 201.5 |
Uterus Tissue Slide (Adenomyoma) |
11-432-4um |
ProSci |
4 um |
EUR 180.5 |
Uterus Membrane Tumor Lysate |
XBL-11031 |
ProSci |
0.1 mg |
EUR 626.75 |
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
Uterus-Corpus Membrane Lysate |
XBL-11037 |
ProSci |
0.1 mg |
EUR 516.5 |
Description: Human uterus-corpus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-corpus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-corpus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-corpus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
Uterus-Fundus Membrane Lysate |
XBL-11040 |
ProSci |
0.1 mg |
EUR 516.5 |
Description: Human uterus-fundus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-fundus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-fundus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-fundus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal. |
Total RNA from Human Adult Normal Tissue: Uterus: Cervix of uterus |
R1234275-50 |
Biochain |
50 ug |
EUR 178 |
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Total RNA from Human Adult Normal Tissue: Uterus: Corpus of Uterus |
R1234276-10 |
Biochain |
10 ug |
EUR 194 |
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Rabbit UTERUS Y 25 EA* |
41253-2 |
Pel-Freez |
25 EA |
EUR 318.39 |
Total RNA from Lupus: Uterus |
R1236274Lup-50 |
Biochain |
50 ug |
EUR 351 |
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
Uterus Tissue Slide (Endometrioid Adenocarcinoma) |
11-405-10um |
ProSci |
10 um |
EUR 201.5 |
Uterus Tissue Slide (Endometrioid Adenocarcinoma) |
11-405-4um |
ProSci |
4 um |
EUR 180.5 |
CAT Antibody |
36312-100ul |
SAB |
100ul |
EUR 252 |
CAT Antibody |
1-CSB-PA070043 |
Cusabio |
|
|
|
Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/20000 |
CAT Antibody |
1-CSB-PA599838 |
Cusabio |
|
|
|
Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:50-1:200 |
CAT Antibody |
1-CSB-PA942303 |
Cusabio |
|
|
|
Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:50-1:200 |
CAT Antibody |
1-CSB-PA004980 |
Cusabio |
|
|
|
Description: A polyclonal antibody against CAT. Recognizes CAT from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000 |
CAT Antibody |
1-CSB-PA004563GA01HU |
Cusabio |
|
|
|
Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC |
CAT antibody |
10R-3584 |
Fitzgerald |
100 ul |
EUR 726 |
Description: Mouse monoclonal CAT antibody |
CAT antibody |
10R-3585 |
Fitzgerald |
100 ul |
EUR 691 |
Description: Mouse monoclonal CAT antibody |
CAT antibody |
70R-16187 |
Fitzgerald |
50 ul |
EUR 435 |
Description: Rabbit polyclonal CAT antibody |
CAT antibody |
20C-CR1111RP |
Fitzgerald |
500 ul |
EUR 262 |
Description: Rabbit polyclonal CAT antibody |
Cat IgM |
31C-CH0213 |
Fitzgerald |
1 mg |
EUR 392 |
Description: Purified Cat IgM |
Total RNA from Human Adult Normal Tissue 5 Donor Pool: Uterus: Cervix of uterus |
R1234275-P |
Biochain |
50 ug |
EUR 328 |
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA Synthesis SuperMix |
20-abx09801420ulSystems |
Abbexa |
|
- 100 rxns × 20 ul Systems
- 50 rxns × 20 ul Systems
|
|
Evo? cDNA Supermix |
M1168-100 |
Biovision |
|
EUR 381 |
Evo? cDNA Supermix |
M1168-25 |
Biovision |
|
EUR 267 |
Novo? cDNA Supermix |
M1169-100 |
Biovision |
|
EUR 441 |
Novo? cDNA Supermix |
M1169-25 |
Biovision |
|
EUR 289 |
Frozen Tissue Section - Human Tumor: Uterus |
T1235274 |
Biochain |
5 slides |
EUR 338 |
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool. |
Uterus Tissue Slide ( Papillary serous Adenocarcinoma) |
11-407-10um |
ProSci |
10 um |
EUR 201.5 |
Uterus Tissue Slide ( Papillary serous Adenocarcinoma) |
11-407-4um |
ProSci |
4 um |
EUR 180.5 |
Uterus Tissue Slide (Spindle Cell Tumor) |
11-418-10um |
ProSci |
10 um |
EUR 201.5 |
Uterus Tissue Slide (Spindle Cell Tumor) |
11-418-4um |
ProSci |
4 um |
EUR 180.5 |
cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis |
C1634310 |
Biochain |
40 reactions |
EUR 621 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Plant Normal Tissue: cDNA from Plant: Corn |
C1634330 |
Biochain |
40 reactions |
EUR 621 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Plant Normal Tissue: cDNA from Plant: Orange |
C1634340 |
Biochain |
40 reactions |
EUR 621 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Plant Normal Tissue: cDNA from Plant: Potato |
C1634350 |
Biochain |
40 reactions |
EUR 621 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Plant Normal Tissue: cDNA from Plant: Rice |
C1634360 |
Biochain |
40 reactions |
EUR 621 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
cDNA from Plant Normal Tissue: cDNA from Plant: Wheat |
C1634390 |
Biochain |
40 reactions |
EUR 621 |
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes. |
PKA CAT Antibody |
abx147539-100ug |
Abbexa |
100 ug |
EUR 439 |
|
Catalase (CAT) Antibody |
20-abx132110 |
Abbexa |
-
EUR 272.00
-
EUR 592.00
-
EUR 314.00
-
EUR 154.00
-
EUR 230.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Catalase (CAT) Antibody |
20-abx132122 |
Abbexa |
-
EUR 300.00
-
EUR 718.00
-
EUR 384.00
-
EUR 154.00
-
EUR 244.00
|
- 100 ug
- 1 mg
- 200 ug
- 20 ug
- 50 ug
|
|
Catalase (CAT) Antibody |
20-abx125619 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 182.00
-
EUR 314.00
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
Catalase (CAT) Antibody |
20-abx125620 |
Abbexa |
-
EUR 411.00
-
EUR 592.00
-
EUR 182.00
-
EUR 314.00
|
- 100 ul
- 200 ul
- 20 ul
- 50 ul
|
|
Catalase (CAT) Antibody |
20-abx111432 |
Abbexa |
|
|
|
CAT Rabbit pAb |
A11777-100ul |
Abclonal |
100 ul |
EUR 308 |
CAT Rabbit pAb |
A11777-200ul |
Abclonal |
200 ul |
EUR 459 |
CAT Rabbit pAb |
A11777-20ul |
Abclonal |
20 ul |
EUR 183 |
CAT Rabbit pAb |
A11777-50ul |
Abclonal |
50 ul |
EUR 223 |
We used this annotation to scale back the bioinformatic complexity related to multi-mapping reads from short-read RNA-seq experiments, and we present that this enchancment is expanded in a TE-rich genome resembling maize. Our TE annotation additionally permits the testing of particular standing hypotheses within the TE subject. We display that wrong TE splicing doesn’t set off small RNA manufacturing, and the cell extra strongly targets DNA methylation to TEs which have the potential to make mRNAs. This work gives a brand new transcript-based TE annotation for Arabidopsis and maize, which serves as a blueprint to scale back the bioinformatic complexity related to repetitive TEs in any organism.