Extraction of high-quality tissue-specific RNA from London plane trees (Platanus acerifolia), permitting the construction of a female inflorescence cDNA library

Extraction of high-quality tissue-specific RNA from London plane trees (Platanus acerifolia), permitting the construction of a female inflorescence cDNA library

The London airplane tree (Platanus acerifolia Willd.) has world significance as an city landscaping tree and is the topic of genetic-improvement applications for productive sterility, illness and/or insect resistance. Molecular evaluation methods are essential to such applications, however could also be impeded by particular difficulties encountered throughout nucleic acid isolation.

An in depth RNA isolation and purification protocol, based mostly on established cetyltrimethyl-ammonium bromide (CTAB) extraction methods mixed with further purification steps utilizing butanol and the ionic detergent CTAB, which overcomes these issues within the woody species P. acerifolia, was performed. In brief, phenolic compounds are certain to soluble polyvinylpyrrolidone after which separated out via LiCl precipitation of the RNA. Subsequently, protein- and carbohydrate-contaminants are eliminated by chloroform partitioning adopted by LiCl-mediated precipitation.

The ensuing isolates of RNA have been discovered to be of enough high quality for profitable use in reverse transcription PCR evaluation. Moreover, RNA isolates from feminine inflorescences have been used for the development of a cDNA library. This library was discovered to comprise a number of full-length cDNA clones of MADS-box genes, according to the library being consultant of inflorescence expression profiles.

Molecular evaluation of a stress-induced cDNA encoding the interpretation initiation issue, eIF1, from the salt-tolerant wild relative of rice, Porteresia coarctata

The evaluation of plant response to emphasize is a crucial path to the invention of genes conferring stress tolerance. Protein synthesis may be very delicate to salt stress and proteins concerned on this course of could also be an necessary determinant of salt tolerance. The halophytic plant, Porteresia coarctata Tateoka, is an in depth relative of Oryza sativa L., and has the power to face up to sudden adjustments within the soil salinity.

The interpretation initiation issue 1 (PceIF1) cDNA was remoted from the leaves of P. coarctata that had been subjected to a high-salt remedy (150 mm NaCl). An expression research confirmed that the abundance of eIF1 transcripts elevated to a most degree 5 d after stress induction after which decreased to ranges much like leaves of management (unsalinised) crops.

This gene was additionally up-regulated in exogenous abscisic acid (ABA) and mannitol remedies, suggesting that its induction is expounded to the water deficit impact of excessive salt. Our research confirmed that expression ranges of eIF1 transcripts may kind a handy indicator for monitoring a stress-responsive mechanism that operates within the leaves of P. coarctata.

Transcriptome evaluation of leaf tissue from Bermudagrass (Cynodon dactylon) utilizing a normalised cDNA library

A normalised cDNA library was constructed from Bermudagrass to achieve perception into the transcriptome of Cynodon dactylon L. A complete of 15 588 high-quality expressed sequence tags (ESTs) from the cDNA library have been subjected to The Institute for Genomic Analysis Gene Indices clustering instruments to supply a unigene set. A complete of 9414 unigenes have been obtained from the high-quality ESTs and solely 39.6% of the high-quality ESTs have been redundant, indicating that the normalisation process was efficient.

A big-scale comparative genomic evaluation of the unigenes was carried out utilizing publicly out there instruments, resembling BLAST, InterProScan and Gene Ontology. The unigenes have been additionally subjected to a seek for EST-derived easy sequence repeats (EST-SSRs) and conserved-intron scanning primers (CISPs), that are helpful as DNA markers.

Extraction of high-quality tissue-specific RNA from London plane trees (Platanus acerifolia), permitting the construction of a female inflorescence cDNA library

Though the candidate EST-SSRs and CISPs discovered within the current research have to be empirically examined, they’re anticipated to be helpful as DNA markers for a lot of functions, together with comparative genomic research of grass species, by advantage of their vital similarities to EST sequences from different grasses. Thus, information of Cynodon ESTs will empower turfgrass analysis by offering homologues for genes which can be thought to confer necessary capabilities in different crops.

Lengthy-read cDNA Sequencing Permits a ‘Gene-Like’ Transcript Annotation of Transposable Parts

Transcript-based annotations of genes facilitate each genome-wide analyses and detailed single locus analysis. In distinction, transposable factor (TE) annotations are rudimentary, consisting of knowledge solely on TE location and sort. The repetitiveness and restricted annotation of TEs prevents the power to differentiate between probably practical expressed parts and degraded copies.

To enhance genome-wide TE bioinformatics, we carried out long-read sequencing of cDNAs from Arabidopsis thaliana traces poor in a number of layers of TE repression. These uniquely-mapping transcripts have been used to determine the set of TEs in a position to generate polyadenylated RNAs and create a brand new transcript-based annotation of TEs that we’ve layered upon the prevailing high-quality group commonplace annotation.

cDNA - Monkey (Rhesus) Normal Tissue: Uterus: Corpus

C1534276 40 reactions
EUR 578

cDNA - Monkey (Rhesus) Normal Tissue: Uterus: Fundus

C1534278 40 reactions
EUR 578

cDNA - Monkey (Cynomolgus) Normal Tissue: Uterus: Cervix

C1534275-Cy 40 reactions
EUR 578

Total RNA - Human Adult Normal Tissue: Uterus: Cervix of uterus

R1234275-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Uterus: Corpus of Uterus

R1234276-10 10 ug
EUR 191

Fetal Uterus Lysate

XBL-10431 0.1 mg
EUR 632.4
Description: Fetal human uterus tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human uterus tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the uterus tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The uterus tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Uterus Membrane Lysate

XBL-11023 0.1 mg
EUR 619.8
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Bovine UTERUS 5 EA*

57153-2 5 EA
EUR 245.04

Total RNA - Lupus: Uterus

R1236274Lup-50 50 ug
EUR 421.2

Human Uterus Tumor lysate

HTL-1384 1 mg
EUR 927.6

Uterus tumor tissue array

UT802a each
EUR 306
Description: Uterus tumor tissue array, including stromal and leiomyo sarcoma, endometrioid adenocarcinoma, chorionepithelioma, hydatidiform mole, clear cell carcinoma, carcinsarcoma, sarcomatoid carcinoma, with pathology grade, TNM/Stage (AJCC 8th edition), 80 cases/80 cores (cores size 1.5mm), replacing UT802

Rabbit UTERUS Y 25 EA*

41253-2 25 EA
EUR 382.07

Uterus Tissue Slide (Normal)

11-401-10um 10 um
EUR 241.8

Uterus Tissue Slide (Normal)

11-401-4um 4 um
EUR 216.6

Uterus Tissue Slide (Benign)

11-402-10um 10 um
EUR 241.8

Uterus Tissue Slide (Benign)

11-402-4um 4 um
EUR 216.6

Total RNA - Human Adult Normal Tissue 5 Donor Pool: Uterus: Cervix of uterus

R1234275-P 50 ug
EUR 370

Uterus Tissue Slide (Abnormal)

11-419-10um 10 um
EUR 241.8

Uterus Tissue Slide (Abnormal)

11-419-4um 4 um
EUR 216.6

Uterus Membrane Tumor Lysate

XBL-11031 0.1 mg
EUR 752.1
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Uterus Tissue Slide (Adenomyoma)

11-432-10um 10 um
EUR 241.8

Uterus Tissue Slide (Adenomyoma)

11-432-4um 4 um
EUR 216.6

Uterus-Corpus Membrane Lysate

XBL-11037 0.1 mg
EUR 619.8
Description: Human uterus-corpus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-corpus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-corpus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-corpus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Uterus-Fundus Membrane Lysate

XBL-11040 0.1 mg
EUR 619.8
Description: Human uterus-fundus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-fundus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-fundus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-fundus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Uterus Tissue Slide (Adenocarcinoma)

11-404-10um 10 um
EUR 241.8

Uterus Tissue Slide (Adenocarcinoma)

11-404-4um 4 um
EUR 216.6

Uterus cancer test tissue array

T093 each
EUR 78
Description: Uterus cancer test tissue array, with normal endometrial tissue as control, including TNM, clinical stage and pathology grade, 2 serial sections, 6 cases/24 cores

Leiomyoma of uterus tissue array

SO806 each
EUR 270
Description: Leiomyoma of uterus tissue array, 80 cases/80 cores

Total RNA - Human Tumor Tissue: Uterus

R1235274-50 50 ug
EUR 400

Uterus Tissue Slide (Spindle Cell Tumor)

11-418-10um 10 um
EUR 241.8

Uterus Tissue Slide (Spindle Cell Tumor)

11-418-4um 4 um
EUR 216.6

Total Protein - Human Tumor Tissue: Uterus

P1235274 1 mg
EUR 310

Frozen Tissue Section - Human Tumor: Uterus

T1235274 5 slides
EUR 330

Membrane Protein - Human Tumor Tissue: Uterus

P3235274 0.1 mg
EUR 410

Uterus Tissue Slide (Endometrioid Adenocarcinoma)

11-405-10um 10 um
EUR 241.8

Uterus Tissue Slide (Endometrioid Adenocarcinoma)

11-405-4um 4 um
EUR 216.6

FFPE Genomic DNA - Human Tumor Tissue: Uterus

D2235274 2 ug
EUR 960

Total RNA - Human Adult Normal Tissue: Uterus

R1234274-50 50 ug
EUR 170

Human Adult Uterus (Normal) Whole tissue lysate

HAL-1317 1 mg
EUR 628.8

Monkey (Cyno.) Normal Uterus Whole tissue lysate

MCL-1222 1 mg
EUR 927.6

Total Protein - Human Adult Normal Tissue: Uterus

P1234274 1 mg
EUR 188

Monkey (Rhesus) Normal Uterus Whole tissue lysate

MRL-1332 1 mg
EUR 927.6

Frozen Tissue Section - Human Adult Normal: Uterus

T1234274 5 slides
EUR 343

Genomic DNA - Lupus: Uterus, from a single donor

D1236274Lup 50 ug
EUR 504

Human Uterus Tissue Slide (Normal) (5 slides/pk)

HTS-11401 1 pk
EUR 343.2

Human Uterus Tissue Slide (Benign) (5 slides/pk)

HTS-11402 1 pk
EUR 343.2

Uterus Tissue Slide ( Papillary serous Adenocarcinoma)

11-407-10um 10 um
EUR 241.8

Uterus Tissue Slide ( Papillary serous Adenocarcinoma)

11-407-4um 4 um
EUR 216.6

Membrane Protein - Human Adult Normal Tissue: Uterus

P3234274 0.1 mg
EUR 270

Paraffin Tissue Section - Human Uterus Cervix Tumor

T2235275 5 slides
EUR 238

Paraffin Tissue Section - Human Adult Normal: Uterus

T2234274 5 slides
EUR 185

Human Uterus Tissue Slide (Abnormal) (5 slides/pk)

HTS-11419 1 pk
EUR 343.2

Human Uterus Tissue Slide (Adenomyoma) (5 slides/pk)

HTS-11432 1 pk
EUR 343.2

Human Uterus Tissue Slide (Adenocarcinoma) (5 slides/pk)

HTS-11404 1 pk
EUR 343.2

Total Protein - Human Adult Normal Tissue: Uterus: Cervix

P1234275 1 mg
EUR 188

Total Protein - Human Adult Normal Tissue: Uterus: Corpus

P1234276 1 mg
EUR 188

Total Protein - Human Adult Normal Tissue: Uterus: Fundus

P1234278 1 mg
EUR 188

Multiple of uterus cancer with endometrium tissue array

UT1501a each
EUR 258
Description: Multiple of uterus cancer with endometrium tissue array, including pathology grade, TNM and clinical stage, 75 cases/150 cores, replacing UT1501

Membrane Protein - Human Adult Normal Tissue: Uterus: Corpus

P3234276 0.1 mg
EUR 270

Membrane Protein - Human Adult Normal Tissue: Uterus: Fundus

P3234278 0.1 mg
EUR 270

Total RNA - Human Adult Normal Tissue 5 Donor Pool: Uterus

R1234274-P 50 ug
EUR 370

Human Uterus Tissue Slide (Spindle cell tumor) (5 slides/pk)

HTS-11418 1 pk
EUR 343.2

Rat Normal Uterus (single section per slide) (5 slides/pack)

RaFPT041 5 slides
EUR 150

Paraffin Tissue Section - Human Uterus Tumor: Adenocarcinoma, MSS

T2235274MSS 5 slides
EUR 524

Normal Human Uterus (single section per slide) (5 slides/pack)

HuFPT096 5 slides
EUR 150

Paraffin Tissue Section - Human Uterus Cervix Tumor, HPV positive

T2235275HPV+ 5 slides
EUR 646.8
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Uterus Cervix Tumor, HPV negative

T2235275HPV- 5 slides
EUR 646.8
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Uterus Cervix Tumor, HPV negative

T2235275HPV-negative 5 slides
EUR 524

Paraffin Tissue Section - Human Uterus Cervix Tumor, HPV positive

T2235275HPV-positive 5 slides
EUR 524

Cynomolgus Monkey: Uterus (single section per slide) (5 slides/pack)

CyFPT081 5 slides
EUR 150

Human Uterus Tissue Slide (Endometrioid Adenocarcinoma) (5 slides/pk)

HTS-11405 1 pk
EUR 343.2

Paraffin Tissue Section - Human Uterus Tumor: Endometrium Adenocarcinoma

T2235274-3 5 slides
EUR 238

Genomic DNA - Human Tumor Tissue: Uterus Tumor, from a single donor

D1235274 50 ug
EUR 489

Genomic DNA - Human Adult Normal Tissue: Uterus, from a single donor

D1234274 100 ug
EUR 286

Human Uterus Tissue Slide (Papillary serous Adenocarcinoma) (5 slides/pk)

HTS-11407 1 pk
EUR 343.2

Genomic DNA - Human Adult Normal Tissue: Uterus: Cervix, from a single donor

D1234275 100 ug
EUR 286

Multiple Tumor Tissue Array - Bladder, Colon, Kidney, Lung, Stomach, and Uterus tumors

T8235716d-2 2 slides
EUR 295

Multiple Tumor Tissue Array - Ovary, Bladder, Breast, Kidney, Uterus, and Lung tumors, plus 12 tumor/normal pairs

T8235714d-2 2 slides
EUR 295

Multiple Tumor Tissue Array - Ovary, Bladder, Breast, Kidney, Uterus, and Lung tumors, plus 12 tumor/normal pairs

T8235714d-5 5 slides
EUR 606

Multiple Tumor Tissue Array - Bladder, Colon, Kidney, Lung, Stomach, and Uterus tumors, plus 12 tumor/normal pairs

T8235716d-5 5 slides
EUR 606

cDNA - Plant Normal Tissue: cDNA - Plant: Corn

C1634330 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Rice

C1634360 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Wheat

C1634390 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Orange

C1634340 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Potato

C1634350 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Arabidopsis

C1634310 40 reactions
EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Soy bean

C1634370 40 reactions
EUR 674

Novo? cDNA Kit

M1165-100 each
EUR 424.8

Novo? cDNA Kit

M1165-25 each
EUR 320.4

cDNA - Lupus: Lung

C1236152Lup 40 reactions
EUR 737

cDNA - Lupus: Colon

C1236090Lup 40 reactions
EUR 737

cDNA - Lupus: Heart

C1236122Lup 40 reactions
EUR 737

cDNA - Lupus: Liver

C1236149Lup 40 reactions
EUR 737

cDNA - Asthma: Lung

C1236152Ld-1 40 reactions
EUR 899

cDNA - Lupus: Kidney

C1236142Lup 40 reactions
EUR 737

cDNA - Lupus: Spleen

C1236246Lup 40 reactions
EUR 737

Evo? cDNA Supermix

M1168-100 each
EUR 457.2

Evo? cDNA Supermix

M1168-25 each
EUR 320.4

cDNA - Lupus: stomach

C1236248Lup 40 reactions
EUR 737

Novo? cDNA Supermix

M1169-100 each
EUR 529.2

Novo? cDNA Supermix

M1169-25 each
EUR 346.8

cDNA - Emphysema: Lung

C1236152Ld-3 40 reactions
EUR 899

cDNA - Pneumonia: Lung

C1236152Ld-4 40 reactions
EUR 899

cDNA - Lupus: Pancreas

C1236188Lup 40 reactions
EUR 737

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems
  • EUR 744.00
  • EUR 627.60
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems
  • EUR 1045.20
  • EUR 794.40
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

cDNA - Bronchitis: Lung

C1236152Ld-2 40 reactions
EUR 899

cDNA - hypertension: Vein

C1236020Hd-2 40 reactions
EUR 729

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 678.00
  • EUR 577.20
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems

cDNA - hypertension: Heart

C1236122Hd-2 40 reactions
EUR 729

Evo™ cDNA Kit

M1164-100 each Ask for price

Evo™ cDNA Kit

M1164-25 each Ask for price

cDNA - hypertension: Artery

C1236013Hd-2 40 reactions
EUR 729

cDNA - hypertension: Kidney

C1236142Hd-2 40 reactions
EUR 729

cDNA - Arteriosclerosis: Aorta

C1236012Hd-4 40 reactions
EUR 899

cDNA - Arteriosclerosis: Artery

C1236013Hd-4 40 reactions
EUR 899

Human eNOS cDNA probe

eNOS51-D-2 2 ug
EUR 534

cDNA - Lupus: Spinal Cord

C1236234Lup 40 reactions
EUR 737

Tetro cDNA Synthesis Kit

BIO-65042 30 Reactions Ask for price

Tetro cDNA Synthesis Kit

BIO-65043 100 Reactions Ask for price

EVery cDNA Synthesis Kit

EVery200B-1 20 preps
EUR 307

cDNA - Liver Cirrhosis: Lung

C1236152Lcs 40 reactions
EUR 729

cDNA - Liver Cirrhosis: Skin

C1236218Lcs 40 reactions
EUR 729

cDNA - Liver Cirrhosis: Brain

C1236035Lcs 40 reactions
EUR 729

cDNA - Liver Cirrhosis: Colon

C1236090Lcs 40 reactions
EUR 729

cDNA - Liver Cirrhosis: Heart

C1236122Lcs 40 reactions
EUR 729

cDNA - Liver Cirrhosis: Liver

C1236149Lcs 40 reactions
EUR 729

cDNA - Lupus: Small Intestine

C1236226Lup 40 reactions
EUR 737

circRNA cDNA Synthesis Kit

G627 25 rxn (20 ul/rxn)
EUR 370.8

cDNA Probe Diluent Solution

AR0063 5mL
EUR 127.2

cDNA - Liver Cirrhosis: Kidney

C1236142Lcs 40 reactions
EUR 729

cDNA - Liver Cirrhosis: Spleen

C1236246Lcs 40 reactions
EUR 729

Novo? Transcriptome cDNA Kit

M1167-100 each
EUR 1142.4

Novo? Transcriptome cDNA Kit

M1167-25 each
EUR 529.2

SensiFAST cDNA Synthesis Kit

BIO-65053 50 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053/S Sample Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65054 250 Reactions Ask for price

cDNA - Alzheimer's Disease: Brain

C1236035Alz 40 reactions
EUR 729

cDNA - Parkinson's Disease: Brain

C1236035Par 40 reactions
EUR 729

cDNA - Pulmonary Embolism: Lung

C1236152Ld-5 40 reactions
EUR 899

cDNA - Liver Cirrhosis: Pancreas

C1236188Lcs 40 reactions
EUR 729

Plant Tissue cDNA: Arabidopsis

PC34-310 10 rxn
EUR 498

cDNA - Liver Cirrhosis: Esophagus

C1236106Lcs 40 reactions
EUR 729

cDNA - Liver Cirrhosis: Diaphragm

C1236169Lcs 40 reactions
EUR 729

cDNA - Dementia: Brain: Hippocampus

C1236052Dem 40 reactions
EUR 886

OneScriptPlus cDNA Synthesis Kit

G235 25 x 20 ul reactions
EUR 116.4

OneScriptPlus cDNA Synthesis Kit

G236 100 x 20 ul reactions
EUR 202.8

cDNA - Depression: Brain: Hippocampus

C1236052Dep 40 reactions
EUR 886

Evo? cDNA Kit (gDNA Removal)

M1166-100 each
EUR 457.2

cDNA - Lupus: Liver: Left Lobe

C1236150Lup 40 reactions
EUR 737

cDNA - Rat Normal Tissue: Lung

C1434152 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Lung

C1734152 40 reactions
EUR 385

cDNA - Lupus: Liver: Right Lobe

C1236151Lup 40 reactions
EUR 737

cDNA - Rat Normal Tissue: Brain

C1434035 40 reactions
EUR 385

cDNA - Rat Normal Tissue: Colon

C1434090 40 reactions
EUR 578

cDNA - Rat Normal Tissue: Heart

C1434122 40 reactions
EUR 385

cDNA - Rat Normal Tissue: Liver

C1434149 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Brain

C1734035 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Cecum

C1734089 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Heart

C1734122 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Liver

C1734149 40 reactions
EUR 385

cDNA - Human Tumor Tissue: Bone

C1235023-10 10 reactions
EUR 274

cDNA - Human Tumor Tissue: Lung

C1235152 40 reactions
EUR 578

cDNA - Human Tumor Tissue: Nose

C1235178-10 10 reactions
EUR 274

cDNA - Human Tumor Tissue: Skin

C1235218-10 10 reactions
EUR 274

Human Adult cDNA Tissue: Lung

HA-152 10 rxn
EUR 498

Human Adult cDNA Tissue: Skin

HA-218 10 rxn
EUR 498

OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 116.4

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 202.8

cDNA - Rat Normal Tissue: Kidney

C1434142 40 reactions
EUR 385

cDNA - Rat Normal Tissue: Rectum

C1434206 40 reactions
EUR 578

cDNA - Rat Normal Tissue: Spleen

C1434246 40 reactions
EUR 385

cDNA - Rat Normal Tissue: Testis

C1434260 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Kidney

C1734142 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Rectum

C1734206 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Testis

C1734260 40 reactions
EUR 385

cDNA - Mouse Normal Tissue: Lung

C1334152 40 reactions
EUR 385

cDNA - Human Tumor Tissue: Brain

C1235035-10 10 reactions
EUR 274

cDNA - Human Tumor Tissue: Colon

C1235090 40 reactions
EUR 578

cDNA - Human Tumor Tissue: Liver

C1235149 40 reactions
EUR 578

cDNA - Human Tumor Tissue: Ovary

C1235183-10 10 reactions
EUR 525

cDNA - Rat Normal Tissue: Adipose

C1434003 40 reactions
EUR 578

cDNA - Rat Normal Tissue: Bladder

C1434010 40 reactions
EUR 385

cDNA - Rat Normal Tissue: Stomach

C1434248 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Bladder

C1734010 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Trachea

C1734160 40 reactions
EUR 385

cDNA - Dog Normal Tissue: Stomach

C1734248 40 reactions
EUR 385

cDNA - Mouse Normal Tissue: Brain

C1334035 40 reactions
EUR 385

We used this annotation to scale back the bioinformatic complexity related to multi-mapping reads from short-read RNA-seq experiments, and we present that this enchancment is expanded in a TE-rich genome resembling maize. Our TE annotation additionally permits the testing of particular standing hypotheses within the TE subject. We display that wrong TE splicing doesn’t set off small RNA manufacturing, and the cell extra strongly targets DNA methylation to TEs which have the potential to make mRNAs. This work gives a brand new transcript-based TE annotation for Arabidopsis and maize, which serves as a blueprint to scale back the bioinformatic complexity related to repetitive TEs in any organism.

Skriv et svar

Din e-mailadresse vil ikke blive publiceret.