Extraction of high-quality tissue-specific RNA from London plane trees (Platanus acerifolia), permitting the construction of a female inflorescence cDNA library

The London airplane tree (Platanus acerifolia Willd.) has world significance as an city landscaping tree and is the topic of genetic-improvement applications for productive sterility, illness and/or insect resistance. Molecular evaluation methods are essential to such applications, however could also be impeded by particular difficulties encountered throughout nucleic acid isolation.

An in depth RNA isolation and purification protocol, based mostly on established cetyltrimethyl-ammonium bromide (CTAB) extraction methods mixed with further purification steps utilizing butanol and the ionic detergent CTAB, which overcomes these issues within the woody species P. acerifolia, was performed. In brief, phenolic compounds are certain to soluble polyvinylpyrrolidone after which separated out via LiCl precipitation of the RNA. Subsequently, protein- and carbohydrate-contaminants are eliminated by chloroform partitioning adopted by LiCl-mediated precipitation.

The ensuing isolates of RNA have been discovered to be of enough high quality for profitable use in reverse transcription PCR evaluation. Moreover, RNA isolates from feminine inflorescences have been used for the development of a cDNA library. This library was discovered to comprise a number of full-length cDNA clones of MADS-box genes, according to the library being consultant of inflorescence expression profiles.

Molecular evaluation of a stress-induced cDNA encoding the interpretation initiation issue, eIF1, from the salt-tolerant wild relative of rice, Porteresia coarctata

The evaluation of plant response to emphasize is a crucial path to the invention of genes conferring stress tolerance. Protein synthesis may be very delicate to salt stress and proteins concerned on this course of could also be an necessary determinant of salt tolerance. The halophytic plant, Porteresia coarctata Tateoka, is an in depth relative of Oryza sativa L., and has the power to face up to sudden adjustments within the soil salinity.

The interpretation initiation issue 1 (PceIF1) cDNA was remoted from the leaves of P. coarctata that had been subjected to a high-salt remedy (150 mm NaCl). An expression research confirmed that the abundance of eIF1 transcripts elevated to a most degree 5 d after stress induction after which decreased to ranges much like leaves of management (unsalinised) crops.

This gene was additionally up-regulated in exogenous abscisic acid (ABA) and mannitol remedies, suggesting that its induction is expounded to the water deficit impact of excessive salt. Our research confirmed that expression ranges of eIF1 transcripts may kind a handy indicator for monitoring a stress-responsive mechanism that operates within the leaves of P. coarctata.

Transcriptome evaluation of leaf tissue from Bermudagrass (Cynodon dactylon) utilizing a normalised cDNA library

A normalised cDNA library was constructed from Bermudagrass to achieve perception into the transcriptome of Cynodon dactylon L. A complete of 15 588 high-quality expressed sequence tags (ESTs) from the cDNA library have been subjected to The Institute for Genomic Analysis Gene Indices clustering instruments to supply a unigene set. A complete of 9414 unigenes have been obtained from the high-quality ESTs and solely 39.6% of the high-quality ESTs have been redundant, indicating that the normalisation process was efficient.

A big-scale comparative genomic evaluation of the unigenes was carried out utilizing publicly out there instruments, resembling BLAST, InterProScan and Gene Ontology. The unigenes have been additionally subjected to a seek for EST-derived easy sequence repeats (EST-SSRs) and conserved-intron scanning primers (CISPs), that are helpful as DNA markers.

Though the candidate EST-SSRs and CISPs discovered within the current research have to be empirically examined, they’re anticipated to be helpful as DNA markers for a lot of functions, together with comparative genomic research of grass species, by advantage of their vital similarities to EST sequences from different grasses. Thus, information of Cynodon ESTs will empower turfgrass analysis by offering homologues for genes which can be thought to confer necessary capabilities in different crops.

Lengthy-read cDNA Sequencing Permits a ‘Gene-Like’ Transcript Annotation of Transposable Parts

Transcript-based annotations of genes facilitate each genome-wide analyses and detailed single locus analysis. In distinction, transposable factor (TE) annotations are rudimentary, consisting of knowledge solely on TE location and sort. The repetitiveness and restricted annotation of TEs prevents the power to differentiate between probably practical expressed parts and degraded copies.

To enhance genome-wide TE bioinformatics, we carried out long-read sequencing of cDNAs from Arabidopsis thaliana traces poor in a number of layers of TE repression. These uniquely-mapping transcripts have been used to determine the set of TEs in a position to generate polyadenylated RNAs and create a brand new transcript-based annotation of TEs that we’ve layered upon the prevailing high-quality group commonplace annotation.

cDNA from Monkey (Cynomolgus) Normal Tissue: Uterus: Cervix
C1534275-Cy	Biochain	40 reactions	EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Uterus: Corpus
C1534276	Biochain	40 reactions	EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Monkey (Rhesus) Normal Tissue: Uterus: Fundus
C1534278	Biochain	40 reactions	EUR 540
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human Catalase (CAT) ELISA Kit
DLR-CAT-Hu-48T	DL Develop	48T	EUR 441
Description: A sandwich quantitative ELISA assay kit for detection of Human Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Catalase (CAT) ELISA Kit
DLR-CAT-Hu-96T	DL Develop	96T	EUR 570
Description: A sandwich quantitative ELISA assay kit for detection of Human Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Catalase (CAT) ELISA Kit
DLR-CAT-Mu-48T	DL Develop	48T	EUR 527
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Mouse Catalase (CAT) ELISA Kit
DLR-CAT-Mu-96T	DL Develop	96T	EUR 688
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Catalase (CAT) ELISA Kit
DLR-CAT-Ra-48T	DL Develop	48T	EUR 549
Description: A sandwich quantitative ELISA assay kit for detection of Rat Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Rat Catalase (CAT) ELISA Kit
DLR-CAT-Ra-96T	DL Develop	96T	EUR 718
Description: A sandwich quantitative ELISA assay kit for detection of Rat Catalase (CAT) in samples from serum, plasma, tissue homogenates or other biological fluids.

Human Catalase (CAT) ELISA Kit
RD-CAT-Hu-48Tests	Reddot Biotech	48 Tests	EUR 436

Human Catalase (CAT) ELISA Kit
RD-CAT-Hu-96Tests	Reddot Biotech	96 Tests	EUR 601

Mouse Catalase (CAT) ELISA Kit
RD-CAT-Mu-48Tests	Reddot Biotech	48 Tests	EUR 533

Mouse Catalase (CAT) ELISA Kit
RD-CAT-Mu-96Tests	Reddot Biotech	96 Tests	EUR 740

Rat Catalase (CAT) ELISA Kit
RD-CAT-Ra-48Tests	Reddot Biotech	48 Tests	EUR 557

Rat Catalase (CAT) ELISA Kit
RD-CAT-Ra-96Tests	Reddot Biotech	96 Tests	EUR 775

Human Catalase (CAT) ELISA Kit
RDR-CAT-Hu-48Tests	Reddot Biotech	48 Tests	EUR 455

Human Catalase (CAT) ELISA Kit
RDR-CAT-Hu-96Tests	Reddot Biotech	96 Tests	EUR 629

Mouse Catalase (CAT) ELISA Kit
RDR-CAT-Mu-48Tests	Reddot Biotech	48 Tests	EUR 557

Mouse Catalase (CAT) ELISA Kit
RDR-CAT-Mu-96Tests	Reddot Biotech	96 Tests	EUR 774

Rat Catalase (CAT) ELISA Kit
RDR-CAT-Ra-48Tests	Reddot Biotech	48 Tests	EUR 583

Rat Catalase (CAT) ELISA Kit
RDR-CAT-Ra-96Tests	Reddot Biotech	96 Tests	EUR 811

Uterus Lysate
1317	ProSci	0.1 mg	EUR 191
Description: Uterus tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Uterus Tumor Lysate
1384	ProSci	0.1 mg	EUR 254
Description: Uterus tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Fetal Uterus Lysate
XBL-10431	ProSci	0.1 mg	EUR 527
Description: Fetal human uterus tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human uterus tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the uterus tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The uterus tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Uterus Membrane Lysate
XBL-11023	ProSci	0.1 mg	EUR 516.5
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Bovine UTERUS 5 EA*
57153-2	Pel-Freez	5 EA	EUR 204.2

Human Uterus Tumor lysate
HTL-1384	Alpha Diagnostics	1 mg	EUR 773

Uterus Tissue Slide (Normal)
11-401-10um	ProSci	10 um	EUR 201.5

Uterus Tissue Slide (Normal)
11-401-4um	ProSci	4 um	EUR 180.5

Uterus Tissue Slide (Benign)
11-402-10um	ProSci	10 um	EUR 201.5

Uterus Tissue Slide (Benign)
11-402-4um	ProSci	4 um	EUR 180.5

Uterus Tissue Slide (Adenocarcinoma)
11-404-10um	ProSci	10 um	EUR 201.5

Uterus Tissue Slide (Adenocarcinoma)
11-404-4um	ProSci	4 um	EUR 180.5

Uterus Tissue Slide (Abnormal)
11-419-10um	ProSci	10 um	EUR 201.5

Uterus Tissue Slide (Abnormal)
11-419-4um	ProSci	4 um	EUR 180.5

Uterus Tissue Slide (Adenomyoma)
11-432-10um	ProSci	10 um	EUR 201.5

Uterus Tissue Slide (Adenomyoma)
11-432-4um	ProSci	4 um	EUR 180.5

Uterus Membrane Tumor Lysate
XBL-11031	ProSci	0.1 mg	EUR 626.75
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Uterus-Corpus Membrane Lysate
XBL-11037	ProSci	0.1 mg	EUR 516.5
Description: Human uterus-corpus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-corpus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-corpus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-corpus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Uterus-Fundus Membrane Lysate
XBL-11040	ProSci	0.1 mg	EUR 516.5
Description: Human uterus-fundus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-fundus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-fundus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-fundus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Total RNA from Human Adult Normal Tissue: Uterus: Cervix of uterus
R1234275-50	Biochain	50 ug	EUR 178
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Total RNA from Human Adult Normal Tissue: Uterus: Corpus of Uterus
R1234276-10	Biochain	10 ug	EUR 194
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

CAT/ Rat CAT ELISA Kit
ELA-E0242r	Lifescience Market	96 Tests	EUR 886

Rabbit UTERUS Y 25 EA*
41253-2	Pel-Freez	25 EA	EUR 318.39

Total RNA from Lupus: Uterus
R1236274Lup-50	Biochain	50 ug	EUR 351
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Uterus Tissue Slide (Endometrioid Adenocarcinoma)
11-405-10um	ProSci	10 um	EUR 201.5

Uterus Tissue Slide (Endometrioid Adenocarcinoma)
11-405-4um	ProSci	4 um	EUR 180.5

CAT Antibody
36312-100ul	SAB	100ul	EUR 252

CAT siRNA
20-abx900807	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

CAT siRNA
20-abx910366	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

CAT siRNA
20-abx910367	Abbexa	EUR 551.00 EUR 732.00	15 nmol 30 nmol

CAT Antibody
1-CSB-PA070043	Cusabio	EUR 222.00 EUR 195.00	100ug 50ug
Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: IHC, ELISA;IHC:1/100-1/300.ELISA:1/20000

CAT Antibody
1-CSB-PA599838	Cusabio	EUR 317.00 EUR 244.00	100ul 50ul
Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:50-1:200

CAT Antibody
1-CSB-PA942303	Cusabio	EUR 317.00 EUR 244.00	100ul 50ul
Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC;ELISA:1:1000-1:2000, WB:1:200-1:1000, IHC:1:50-1:200

CAT Antibody
1-CSB-PA004980	Cusabio	EUR 222.00 EUR 195.00	100ug 50ug
Description: A polyclonal antibody against CAT. Recognizes CAT from Human. This antibody is Unconjugated. Tested in the following application: WB, ELISA;WB:1/500-1/2000.ELISA:1/20000

CAT Antibody
1-CSB-PA004563GA01HU	Cusabio	EUR 597.00 EUR 333.00	150ul 50ul
Description: A polyclonal antibody against CAT. Recognizes CAT from Human, Mouse, Rat. This antibody is Unconjugated. Tested in the following application: ELISA, WB, IHC

pCas9- CAT
PVT11001	Lifescience Market	2 ug	EUR 301

pOTB7-CAT
PVT18217	Lifescience Market	2 ug	EUR 231

CAT antibody
10R-3584	Fitzgerald	100 ul	EUR 726
Description: Mouse monoclonal CAT antibody

CAT antibody
10R-3585	Fitzgerald	100 ul	EUR 691
Description: Mouse monoclonal CAT antibody

CAT antibody
70R-16187	Fitzgerald	50 ul	EUR 435
Description: Rabbit polyclonal CAT antibody

CAT antibody
20C-CR1111RP	Fitzgerald	500 ul	EUR 262
Description: Rabbit polyclonal CAT antibody

Cat IgM
31C-CH0213	Fitzgerald	1 mg	EUR 392
Description: Purified Cat IgM

Total RNA from Human Adult Normal Tissue 5 Donor Pool: Uterus: Cervix of uterus
R1234275-P	Biochain	50 ug	EUR 328
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA Synthesis SuperMix
20-abx09801420ulSystems	Abbexa	EUR 565.00 EUR 481.00	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

Novo? cDNA Kit
M1165-100	Biovision		EUR 354

Novo? cDNA Kit
M1165-25	Biovision		EUR 267

Evo? cDNA Supermix
M1168-100	Biovision		EUR 381

Evo? cDNA Supermix
M1168-25	Biovision		EUR 267

Novo? cDNA Supermix
M1169-100	Biovision		EUR 441

Novo? cDNA Supermix
M1169-25	Biovision		EUR 289

Frozen Tissue Section - Human Tumor: Uterus
T1235274	Biochain	5 slides	EUR 338
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Uterus Tissue Slide ( Papillary serous Adenocarcinoma)
11-407-10um	ProSci	10 um	EUR 201.5

Uterus Tissue Slide ( Papillary serous Adenocarcinoma)
11-407-4um	ProSci	4 um	EUR 180.5

Uterus Tissue Slide (Spindle Cell Tumor)
11-418-10um	ProSci	10 um	EUR 201.5

Uterus Tissue Slide (Spindle Cell Tumor)
11-418-4um	ProSci	4 um	EUR 180.5

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis
C1634310	Biochain	40 reactions	EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn
C1634330	Biochain	40 reactions	EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange
C1634340	Biochain	40 reactions	EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato
C1634350	Biochain	40 reactions	EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice
C1634360	Biochain	40 reactions	EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat
C1634390	Biochain	40 reactions	EUR 621
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

PKA CAT Antibody
abx147539-100ug	Abbexa	100 ug	EUR 439

Catalase (CAT) Antibody
20-abx132110	Abbexa	EUR 272.00 EUR 592.00 EUR 314.00 EUR 154.00 EUR 230.00	100 ug 1 mg 200 ug 20 ug 50 ug

Catalase (CAT) Antibody
20-abx132122	Abbexa	EUR 300.00 EUR 718.00 EUR 384.00 EUR 154.00 EUR 244.00	100 ug 1 mg 200 ug 20 ug 50 ug

Catalase (CAT) Antibody
20-abx125619	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

Catalase (CAT) Antibody
20-abx125620	Abbexa	EUR 411.00 EUR 592.00 EUR 182.00 EUR 314.00	100 ul 200 ul 20 ul 50 ul

Catalase (CAT) Antibody
20-abx111432	Abbexa	EUR 732.00 EUR 398.00	150 ul 50 ul

CAT Rabbit pAb
A11777-100ul	Abclonal	100 ul	EUR 308

CAT Rabbit pAb
A11777-200ul	Abclonal	200 ul	EUR 459

CAT Rabbit pAb
A11777-20ul	Abclonal	20 ul	EUR 183

CAT Rabbit pAb
A11777-50ul	Abclonal	50 ul	EUR 223

We used this annotation to scale back the bioinformatic complexity related to multi-mapping reads from short-read RNA-seq experiments, and we present that this enchancment is expanded in a TE-rich genome resembling maize. Our TE annotation additionally permits the testing of particular standing hypotheses within the TE subject. We display that wrong TE splicing doesn’t set off small RNA manufacturing, and the cell extra strongly targets DNA methylation to TEs which have the potential to make mRNAs. This work gives a brand new transcript-based TE annotation for Arabidopsis and maize, which serves as a blueprint to scale back the bioinformatic complexity related to repetitive TEs in any organism.