Extraction of high-quality tissue-specific RNA from London plane trees (Platanus acerifolia), permitting the construction of a female inflorescence cDNA library

The London airplane tree (Platanus acerifolia Willd.) has world significance as an city landscaping tree and is the topic of genetic-improvement applications for productive sterility, illness and/or insect resistance. Molecular evaluation methods are essential to such applications, however could also be impeded by particular difficulties encountered throughout nucleic acid isolation.

An in depth RNA isolation and purification protocol, based mostly on established cetyltrimethyl-ammonium bromide (CTAB) extraction methods mixed with further purification steps utilizing butanol and the ionic detergent CTAB, which overcomes these issues within the woody species P. acerifolia, was performed. In brief, phenolic compounds are certain to soluble polyvinylpyrrolidone after which separated out via LiCl precipitation of the RNA. Subsequently, protein- and carbohydrate-contaminants are eliminated by chloroform partitioning adopted by LiCl-mediated precipitation.

The ensuing isolates of RNA have been discovered to be of enough high quality for profitable use in reverse transcription PCR evaluation. Moreover, RNA isolates from feminine inflorescences have been used for the development of a cDNA library. This library was discovered to comprise a number of full-length cDNA clones of MADS-box genes, according to the library being consultant of inflorescence expression profiles.

Molecular evaluation of a stress-induced cDNA encoding the interpretation initiation issue, eIF1, from the salt-tolerant wild relative of rice, Porteresia coarctata

The evaluation of plant response to emphasize is a crucial path to the invention of genes conferring stress tolerance. Protein synthesis may be very delicate to salt stress and proteins concerned on this course of could also be an necessary determinant of salt tolerance. The halophytic plant, Porteresia coarctata Tateoka, is an in depth relative of Oryza sativa L., and has the power to face up to sudden adjustments within the soil salinity.

The interpretation initiation issue 1 (PceIF1) cDNA was remoted from the leaves of P. coarctata that had been subjected to a high-salt remedy (150 mm NaCl). An expression research confirmed that the abundance of eIF1 transcripts elevated to a most degree 5 d after stress induction after which decreased to ranges much like leaves of management (unsalinised) crops.

This gene was additionally up-regulated in exogenous abscisic acid (ABA) and mannitol remedies, suggesting that its induction is expounded to the water deficit impact of excessive salt. Our research confirmed that expression ranges of eIF1 transcripts may kind a handy indicator for monitoring a stress-responsive mechanism that operates within the leaves of P. coarctata.

Transcriptome evaluation of leaf tissue from Bermudagrass (Cynodon dactylon) utilizing a normalised cDNA library

A normalised cDNA library was constructed from Bermudagrass to achieve perception into the transcriptome of Cynodon dactylon L. A complete of 15 588 high-quality expressed sequence tags (ESTs) from the cDNA library have been subjected to The Institute for Genomic Analysis Gene Indices clustering instruments to supply a unigene set. A complete of 9414 unigenes have been obtained from the high-quality ESTs and solely 39.6% of the high-quality ESTs have been redundant, indicating that the normalisation process was efficient.

A big-scale comparative genomic evaluation of the unigenes was carried out utilizing publicly out there instruments, resembling BLAST, InterProScan and Gene Ontology. The unigenes have been additionally subjected to a seek for EST-derived easy sequence repeats (EST-SSRs) and conserved-intron scanning primers (CISPs), that are helpful as DNA markers.

Though the candidate EST-SSRs and CISPs discovered within the current research have to be empirically examined, they’re anticipated to be helpful as DNA markers for a lot of functions, together with comparative genomic research of grass species, by advantage of their vital similarities to EST sequences from different grasses. Thus, information of Cynodon ESTs will empower turfgrass analysis by offering homologues for genes which can be thought to confer necessary capabilities in different crops.

Lengthy-read cDNA Sequencing Permits a ‘Gene-Like’ Transcript Annotation of Transposable Parts

Transcript-based annotations of genes facilitate each genome-wide analyses and detailed single locus analysis. In distinction, transposable factor (TE) annotations are rudimentary, consisting of knowledge solely on TE location and sort. The repetitiveness and restricted annotation of TEs prevents the power to differentiate between probably practical expressed parts and degraded copies.

To enhance genome-wide TE bioinformatics, we carried out long-read sequencing of cDNAs from Arabidopsis thaliana traces poor in a number of layers of TE repression. These uniquely-mapping transcripts have been used to determine the set of TEs in a position to generate polyadenylated RNAs and create a brand new transcript-based annotation of TEs that we’ve layered upon the prevailing high-quality group commonplace annotation.

cDNA - Monkey (Rhesus) Normal Tissue: Uterus: Corpus
C1534276	Biochain	40 reactions	EUR 578

cDNA - Monkey (Rhesus) Normal Tissue: Uterus: Fundus
C1534278	Biochain	40 reactions	EUR 578

cDNA - Monkey (Cynomolgus) Normal Tissue: Uterus: Cervix
C1534275-Cy	Biochain	40 reactions	EUR 578

Total RNA - Human Adult Normal Tissue: Uterus: Cervix of uterus
R1234275-50	Biochain	50 ug	EUR 170

Total RNA - Human Adult Normal Tissue: Uterus: Corpus of Uterus
R1234276-10	Biochain	10 ug	EUR 191

Fetal Uterus Lysate
XBL-10431	ProSci	0.1 mg	EUR 632.4
Description: Fetal human uterus tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human uterus tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the uterus tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The uterus tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Uterus Membrane Lysate
XBL-11023	ProSci	0.1 mg	EUR 619.8
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Bovine UTERUS 5 EA*
57153-2	Pel-Freez	5 EA	EUR 245.04

Total RNA - Lupus: Uterus
R1236274Lup-50	Biochain	50 ug	EUR 421.2

Human Uterus Tumor lysate
HTL-1384	Alpha Diagnostics	1 mg	EUR 927.6

Uterus tumor tissue array
UT802a	TissueArray	each	EUR 306
Description: Uterus tumor tissue array, including stromal and leiomyo sarcoma, endometrioid adenocarcinoma, chorionepithelioma, hydatidiform mole, clear cell carcinoma, carcinsarcoma, sarcomatoid carcinoma, with pathology grade, TNM/Stage (AJCC 8th edition), 80 cases/80 cores (cores size 1.5mm), replacing UT802

Rabbit UTERUS Y 25 EA*
41253-2	Pel-Freez	25 EA	EUR 382.07

Uterus Tissue Slide (Normal)
11-401-10um	ProSci	10 um	EUR 241.8

Uterus Tissue Slide (Normal)
11-401-4um	ProSci	4 um	EUR 216.6

Uterus Tissue Slide (Benign)
11-402-10um	ProSci	10 um	EUR 241.8

Uterus Tissue Slide (Benign)
11-402-4um	ProSci	4 um	EUR 216.6

Total RNA - Human Adult Normal Tissue 5 Donor Pool: Uterus: Cervix of uterus
R1234275-P	Biochain	50 ug	EUR 370

Uterus Tissue Slide (Abnormal)
11-419-10um	ProSci	10 um	EUR 241.8

Uterus Tissue Slide (Abnormal)
11-419-4um	ProSci	4 um	EUR 216.6

Uterus Membrane Tumor Lysate
XBL-11031	ProSci	0.1 mg	EUR 752.1
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Uterus Tissue Slide (Adenomyoma)
11-432-10um	ProSci	10 um	EUR 241.8

Uterus Tissue Slide (Adenomyoma)
11-432-4um	ProSci	4 um	EUR 216.6

Uterus-Corpus Membrane Lysate
XBL-11037	ProSci	0.1 mg	EUR 619.8
Description: Human uterus-corpus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-corpus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-corpus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-corpus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Uterus-Fundus Membrane Lysate
XBL-11040	ProSci	0.1 mg	EUR 619.8
Description: Human uterus-fundus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus-fundus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus-fundus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus-fundus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Uterus Tissue Slide (Adenocarcinoma)
11-404-10um	ProSci	10 um	EUR 241.8

Uterus Tissue Slide (Adenocarcinoma)
11-404-4um	ProSci	4 um	EUR 216.6

Uterus cancer test tissue array
T093	TissueArray	each	EUR 78
Description: Uterus cancer test tissue array, with normal endometrial tissue as control, including TNM, clinical stage and pathology grade, 2 serial sections, 6 cases/24 cores

Leiomyoma of uterus tissue array
SO806	TissueArray	each	EUR 270
Description: Leiomyoma of uterus tissue array, 80 cases/80 cores

Total RNA - Human Tumor Tissue: Uterus
R1235274-50	Biochain	50 ug	EUR 400

Uterus Tissue Slide (Spindle Cell Tumor)
11-418-10um	ProSci	10 um	EUR 241.8

Uterus Tissue Slide (Spindle Cell Tumor)
11-418-4um	ProSci	4 um	EUR 216.6

Total Protein - Human Tumor Tissue: Uterus
P1235274	Biochain	1 mg	EUR 310

Frozen Tissue Section - Human Tumor: Uterus
T1235274	Biochain	5 slides	EUR 330

Membrane Protein - Human Tumor Tissue: Uterus
P3235274	Biochain	0.1 mg	EUR 410

Uterus Tissue Slide (Endometrioid Adenocarcinoma)
11-405-10um	ProSci	10 um	EUR 241.8

Uterus Tissue Slide (Endometrioid Adenocarcinoma)
11-405-4um	ProSci	4 um	EUR 216.6

FFPE Genomic DNA - Human Tumor Tissue: Uterus
D2235274	Biochain	2 ug	EUR 960

Total RNA - Human Adult Normal Tissue: Uterus
R1234274-50	Biochain	50 ug	EUR 170

Human Adult Uterus (Normal) Whole tissue lysate
HAL-1317	Alpha Diagnostics	1 mg	EUR 628.8

Monkey (Cyno.) Normal Uterus Whole tissue lysate
MCL-1222	Alpha Diagnostics	1 mg	EUR 927.6

Total Protein - Human Adult Normal Tissue: Uterus
P1234274	Biochain	1 mg	EUR 188

Monkey (Rhesus) Normal Uterus Whole tissue lysate
MRL-1332	Alpha Diagnostics	1 mg	EUR 927.6

Frozen Tissue Section - Human Adult Normal: Uterus
T1234274	Biochain	5 slides	EUR 343

Genomic DNA - Lupus: Uterus, from a single donor
D1236274Lup	Biochain	50 ug	EUR 504

Human Uterus Tissue Slide (Normal) (5 slides/pk)
HTS-11401	Alpha Diagnostics	1 pk	EUR 343.2

Human Uterus Tissue Slide (Benign) (5 slides/pk)
HTS-11402	Alpha Diagnostics	1 pk	EUR 343.2

Uterus Tissue Slide ( Papillary serous Adenocarcinoma)
11-407-10um	ProSci	10 um	EUR 241.8

Uterus Tissue Slide ( Papillary serous Adenocarcinoma)
11-407-4um	ProSci	4 um	EUR 216.6

Membrane Protein - Human Adult Normal Tissue: Uterus
P3234274	Biochain	0.1 mg	EUR 270

Paraffin Tissue Section - Human Uterus Cervix Tumor
T2235275	Biochain	5 slides	EUR 238

Paraffin Tissue Section - Human Adult Normal: Uterus
T2234274	Biochain	5 slides	EUR 185

Human Uterus Tissue Slide (Abnormal) (5 slides/pk)
HTS-11419	Alpha Diagnostics	1 pk	EUR 343.2

Human Uterus Tissue Slide (Adenomyoma) (5 slides/pk)
HTS-11432	Alpha Diagnostics	1 pk	EUR 343.2

Human Uterus Tissue Slide (Adenocarcinoma) (5 slides/pk)
HTS-11404	Alpha Diagnostics	1 pk	EUR 343.2

Total Protein - Human Adult Normal Tissue: Uterus: Cervix
P1234275	Biochain	1 mg	EUR 188

Total Protein - Human Adult Normal Tissue: Uterus: Corpus
P1234276	Biochain	1 mg	EUR 188

Total Protein - Human Adult Normal Tissue: Uterus: Fundus
P1234278	Biochain	1 mg	EUR 188

Multiple of uterus cancer with endometrium tissue array
UT1501a	TissueArray	each	EUR 258
Description: Multiple of uterus cancer with endometrium tissue array, including pathology grade, TNM and clinical stage, 75 cases/150 cores, replacing UT1501

Membrane Protein - Human Adult Normal Tissue: Uterus: Corpus
P3234276	Biochain	0.1 mg	EUR 270

Membrane Protein - Human Adult Normal Tissue: Uterus: Fundus
P3234278	Biochain	0.1 mg	EUR 270

Total RNA - Human Adult Normal Tissue 5 Donor Pool: Uterus
R1234274-P	Biochain	50 ug	EUR 370

Human Uterus Tissue Slide (Spindle cell tumor) (5 slides/pk)
HTS-11418	Alpha Diagnostics	1 pk	EUR 343.2

Rat Normal Uterus (single section per slide) (5 slides/pack)
RaFPT041	TissueArray	5 slides	EUR 150

Paraffin Tissue Section - Human Uterus Tumor: Adenocarcinoma, MSS
T2235274MSS	Biochain	5 slides	EUR 524

Normal Human Uterus (single section per slide) (5 slides/pack)
HuFPT096	TissueArray	5 slides	EUR 150

Paraffin Tissue Section - Human Uterus Cervix Tumor, HPV positive
T2235275HPV+	Biochain	5 slides	EUR 646.8
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Uterus Cervix Tumor, HPV negative
T2235275HPV-	Biochain	5 slides	EUR 646.8
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Paraffin Tissue Section - Human Uterus Cervix Tumor, HPV negative
T2235275HPV-negative	Biochain	5 slides	EUR 524

Paraffin Tissue Section - Human Uterus Cervix Tumor, HPV positive
T2235275HPV-positive	Biochain	5 slides	EUR 524

Cynomolgus Monkey: Uterus (single section per slide) (5 slides/pack)
CyFPT081	TissueArray	5 slides	EUR 150

Human Uterus Tissue Slide (Endometrioid Adenocarcinoma) (5 slides/pk)
HTS-11405	Alpha Diagnostics	1 pk	EUR 343.2

Paraffin Tissue Section - Human Uterus Tumor: Endometrium Adenocarcinoma
T2235274-3	Biochain	5 slides	EUR 238

Genomic DNA - Human Tumor Tissue: Uterus Tumor, from a single donor
D1235274	Biochain	50 ug	EUR 489

Genomic DNA - Human Adult Normal Tissue: Uterus, from a single donor
D1234274	Biochain	100 ug	EUR 286

Human Uterus Tissue Slide (Papillary serous Adenocarcinoma) (5 slides/pk)
HTS-11407	Alpha Diagnostics	1 pk	EUR 343.2

Genomic DNA - Human Adult Normal Tissue: Uterus: Cervix, from a single donor
D1234275	Biochain	100 ug	EUR 286

Multiple Tumor Tissue Array - Bladder, Colon, Kidney, Lung, Stomach, and Uterus tumors
T8235716d-2	Biochain	2 slides	EUR 295

Multiple Tumor Tissue Array - Ovary, Bladder, Breast, Kidney, Uterus, and Lung tumors, plus 12 tumor/normal pairs
T8235714d-2	Biochain	2 slides	EUR 295

Multiple Tumor Tissue Array - Ovary, Bladder, Breast, Kidney, Uterus, and Lung tumors, plus 12 tumor/normal pairs
T8235714d-5	Biochain	5 slides	EUR 606

Multiple Tumor Tissue Array - Bladder, Colon, Kidney, Lung, Stomach, and Uterus tumors, plus 12 tumor/normal pairs
T8235716d-5	Biochain	5 slides	EUR 606

cDNA - Plant Normal Tissue: cDNA - Plant: Corn
C1634330	Biochain	40 reactions	EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Rice
C1634360	Biochain	40 reactions	EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Wheat
C1634390	Biochain	40 reactions	EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Orange
C1634340	Biochain	40 reactions	EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Potato
C1634350	Biochain	40 reactions	EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Arabidopsis
C1634310	Biochain	40 reactions	EUR 674

cDNA - Plant Normal Tissue: cDNA - Plant: Soy bean
C1634370	Biochain	40 reactions	EUR 674

Novo? cDNA Kit
M1165-100	Biovision	each	EUR 424.8

Novo? cDNA Kit
M1165-25	Biovision	each	EUR 320.4

cDNA - Lupus: Lung
C1236152Lup	Biochain	40 reactions	EUR 737

cDNA - Lupus: Colon
C1236090Lup	Biochain	40 reactions	EUR 737

cDNA - Lupus: Heart
C1236122Lup	Biochain	40 reactions	EUR 737

cDNA - Lupus: Liver
C1236149Lup	Biochain	40 reactions	EUR 737

cDNA - Asthma: Lung
C1236152Ld-1	Biochain	40 reactions	EUR 899

cDNA - Lupus: Kidney
C1236142Lup	Biochain	40 reactions	EUR 737

cDNA - Lupus: Spleen
C1236246Lup	Biochain	40 reactions	EUR 737

Evo? cDNA Supermix
M1168-100	Biovision	each	EUR 457.2

Evo? cDNA Supermix
M1168-25	Biovision	each	EUR 320.4

cDNA - Lupus: stomach
C1236248Lup	Biochain	40 reactions	EUR 737

Novo? cDNA Supermix
M1169-100	Biovision	each	EUR 529.2

Novo? cDNA Supermix
M1169-25	Biovision	each	EUR 346.8

cDNA - Emphysema: Lung
C1236152Ld-3	Biochain	40 reactions	EUR 899

cDNA - Pneumonia: Lung
C1236152Ld-4	Biochain	40 reactions	EUR 899

cDNA - Lupus: Pancreas
C1236188Lup	Biochain	40 reactions	EUR 737

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)
20-abx09801620ulSystems	Abbexa	EUR 744.00 EUR 627.60	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)
20-abx09802120ulSystems	Abbexa	EUR 1045.20 EUR 794.40	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

cDNA - Bronchitis: Lung
C1236152Ld-2	Biochain	40 reactions	EUR 899

cDNA - hypertension: Vein
C1236020Hd-2	Biochain	40 reactions	EUR 729

cDNA Synthesis SuperMix
20-abx09801420ulSystems	Abbexa	EUR 678.00 EUR 577.20	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

cDNA - hypertension: Heart
C1236122Hd-2	Biochain	40 reactions	EUR 729

Evo™ cDNA Kit
M1164-100	Biovision	each	Ask for price

Evo™ cDNA Kit
M1164-25	Biovision	each	Ask for price

cDNA - hypertension: Artery
C1236013Hd-2	Biochain	40 reactions	EUR 729

cDNA - hypertension: Kidney
C1236142Hd-2	Biochain	40 reactions	EUR 729

cDNA - Arteriosclerosis: Aorta
C1236012Hd-4	Biochain	40 reactions	EUR 899

cDNA - Arteriosclerosis: Artery
C1236013Hd-4	Biochain	40 reactions	EUR 899

Human eNOS cDNA probe
eNOS51-D-2	Alpha Diagnostics	2 ug	EUR 534

cDNA - Lupus: Spinal Cord
C1236234Lup	Biochain	40 reactions	EUR 737

Tetro cDNA Synthesis Kit
BIO-65042	Bioline	30 Reactions	Ask for price

Tetro cDNA Synthesis Kit
BIO-65043	Bioline	100 Reactions	Ask for price

EVery cDNA Synthesis Kit
EVery200B-1	SBI	20 preps	EUR 307

cDNA - Liver Cirrhosis: Lung
C1236152Lcs	Biochain	40 reactions	EUR 729

cDNA - Liver Cirrhosis: Skin
C1236218Lcs	Biochain	40 reactions	EUR 729

cDNA - Liver Cirrhosis: Brain
C1236035Lcs	Biochain	40 reactions	EUR 729

cDNA - Liver Cirrhosis: Colon
C1236090Lcs	Biochain	40 reactions	EUR 729

cDNA - Liver Cirrhosis: Heart
C1236122Lcs	Biochain	40 reactions	EUR 729

cDNA - Liver Cirrhosis: Liver
C1236149Lcs	Biochain	40 reactions	EUR 729

cDNA - Lupus: Small Intestine
C1236226Lup	Biochain	40 reactions	EUR 737

circRNA cDNA Synthesis Kit
G627	ABM	25 rxn (20 ul/rxn)	EUR 370.8

cDNA Probe Diluent Solution
AR0063	BosterBio	5mL	EUR 127.2

cDNA - Liver Cirrhosis: Kidney
C1236142Lcs	Biochain	40 reactions	EUR 729

cDNA - Liver Cirrhosis: Spleen
C1236246Lcs	Biochain	40 reactions	EUR 729

Novo? Transcriptome cDNA Kit
M1167-100	Biovision	each	EUR 1142.4

Novo? Transcriptome cDNA Kit
M1167-25	Biovision	each	EUR 529.2

SensiFAST cDNA Synthesis Kit
BIO-65053	Bioline	50 Reactions	Ask for price

SensiFAST cDNA Synthesis Kit
BIO-65053/S	Bioline	Sample	Ask for price

SensiFAST cDNA Synthesis Kit
BIO-65054	Bioline	250 Reactions	Ask for price

cDNA - Alzheimer's Disease: Brain
C1236035Alz	Biochain	40 reactions	EUR 729

cDNA - Parkinson's Disease: Brain
C1236035Par	Biochain	40 reactions	EUR 729

cDNA - Pulmonary Embolism: Lung
C1236152Ld-5	Biochain	40 reactions	EUR 899

cDNA - Liver Cirrhosis: Pancreas
C1236188Lcs	Biochain	40 reactions	EUR 729

Plant Tissue cDNA: Arabidopsis
PC34-310	Alpha Diagnostics	10 rxn	EUR 498

cDNA - Liver Cirrhosis: Esophagus
C1236106Lcs	Biochain	40 reactions	EUR 729

cDNA - Liver Cirrhosis: Diaphragm
C1236169Lcs	Biochain	40 reactions	EUR 729

cDNA - Dementia: Brain: Hippocampus
C1236052Dem	Biochain	40 reactions	EUR 886

OneScriptPlus cDNA Synthesis Kit
G235	ABM	25 x 20 ul reactions	EUR 116.4

OneScriptPlus cDNA Synthesis Kit
G236	ABM	100 x 20 ul reactions	EUR 202.8

cDNA - Depression: Brain: Hippocampus
C1236052Dep	Biochain	40 reactions	EUR 886

Evo? cDNA Kit (gDNA Removal)
M1166-100	Biovision	each	EUR 457.2

cDNA - Lupus: Liver: Left Lobe
C1236150Lup	Biochain	40 reactions	EUR 737

cDNA - Rat Normal Tissue: Lung
C1434152	Biochain	40 reactions	EUR 385

cDNA - Dog Normal Tissue: Lung
C1734152	Biochain	40 reactions	EUR 385

cDNA - Lupus: Liver: Right Lobe
C1236151Lup	Biochain	40 reactions	EUR 737

cDNA - Rat Normal Tissue: Brain
C1434035	Biochain	40 reactions	EUR 385

cDNA - Rat Normal Tissue: Colon
C1434090	Biochain	40 reactions	EUR 578

cDNA - Rat Normal Tissue: Heart
C1434122	Biochain	40 reactions	EUR 385

cDNA - Rat Normal Tissue: Liver
C1434149	Biochain	40 reactions	EUR 385

cDNA - Dog Normal Tissue: Brain
C1734035	Biochain	40 reactions	EUR 385

cDNA - Dog Normal Tissue: Cecum
C1734089	Biochain	40 reactions	EUR 385

cDNA - Dog Normal Tissue: Heart
C1734122	Biochain	40 reactions	EUR 385

cDNA - Dog Normal Tissue: Liver
C1734149	Biochain	40 reactions	EUR 385

cDNA - Human Tumor Tissue: Bone
C1235023-10	Biochain	10 reactions	EUR 274

cDNA - Human Tumor Tissue: Lung
C1235152	Biochain	40 reactions	EUR 578

cDNA - Human Tumor Tissue: Nose
C1235178-10	Biochain	10 reactions	EUR 274

cDNA - Human Tumor Tissue: Skin
C1235218-10	Biochain	10 reactions	EUR 274

Human Adult cDNA Tissue: Lung
HA-152	Alpha Diagnostics	10 rxn	EUR 498

Human Adult cDNA Tissue: Skin
HA-218	Alpha Diagnostics	10 rxn	EUR 498

OneScriptPlus cDNA Synthesis SuperMix
G453	ABM	25 x 20 ul reactions	EUR 116.4

OneScriptPlus cDNA Synthesis SuperMix
G454	ABM	100 x 20 ul reactions	EUR 202.8

cDNA - Rat Normal Tissue: Kidney
C1434142	Biochain	40 reactions	EUR 385

cDNA - Rat Normal Tissue: Rectum
C1434206	Biochain	40 reactions	EUR 578

cDNA - Rat Normal Tissue: Spleen
C1434246	Biochain	40 reactions	EUR 385

cDNA - Rat Normal Tissue: Testis
C1434260	Biochain	40 reactions	EUR 385

cDNA - Dog Normal Tissue: Kidney
C1734142	Biochain	40 reactions	EUR 385

cDNA - Dog Normal Tissue: Rectum
C1734206	Biochain	40 reactions	EUR 385

cDNA - Dog Normal Tissue: Testis
C1734260	Biochain	40 reactions	EUR 385

cDNA - Mouse Normal Tissue: Lung
C1334152	Biochain	40 reactions	EUR 385

cDNA - Human Tumor Tissue: Brain
C1235035-10	Biochain	10 reactions	EUR 274

cDNA - Human Tumor Tissue: Colon
C1235090	Biochain	40 reactions	EUR 578

cDNA - Human Tumor Tissue: Liver
C1235149	Biochain	40 reactions	EUR 578

cDNA - Human Tumor Tissue: Ovary
C1235183-10	Biochain	10 reactions	EUR 525

cDNA - Rat Normal Tissue: Adipose
C1434003	Biochain	40 reactions	EUR 578

cDNA - Rat Normal Tissue: Bladder
C1434010	Biochain	40 reactions	EUR 385

cDNA - Rat Normal Tissue: Stomach
C1434248	Biochain	40 reactions	EUR 385

cDNA - Dog Normal Tissue: Bladder
C1734010	Biochain	40 reactions	EUR 385

cDNA - Dog Normal Tissue: Trachea
C1734160	Biochain	40 reactions	EUR 385

cDNA - Dog Normal Tissue: Stomach
C1734248	Biochain	40 reactions	EUR 385

cDNA - Mouse Normal Tissue: Brain
C1334035	Biochain	40 reactions	EUR 385

We used this annotation to scale back the bioinformatic complexity related to multi-mapping reads from short-read RNA-seq experiments, and we present that this enchancment is expanded in a TE-rich genome resembling maize. Our TE annotation additionally permits the testing of particular standing hypotheses within the TE subject. We display that wrong TE splicing doesn’t set off small RNA manufacturing, and the cell extra strongly targets DNA methylation to TEs which have the potential to make mRNAs. This work gives a brand new transcript-based TE annotation for Arabidopsis and maize, which serves as a blueprint to scale back the bioinformatic complexity related to repetitive TEs in any organism.