Profiling of rice Cd-tolerant genes through yeast-based cDNA library survival screening

The bioaccumulation of cadmium (Cd) in crop and the following meals chain has aroused intensive issues. Nevertheless, the underlying molecular mechanisms of plant Cd tolerance stay to be clarified from the perspective of novel candidate genes. Right here we described a extremely environment friendly method for preliminary figuring out rice Cd-tolerant genes by way of the yeast-based cDNA library survival screening mixed with high-throughput sequencing technique.

About 690 gene isoforms have been recognized as being Cd-tolerant candidates utilizing this shotgun method. Among the many Cd-tolerant genes recognized, a number of classes of genes akin to BAX inhibitor (BI), NAC transcription elements and Speedy ALkalinization Elements (RALFs) have been of explicit curiosity, and their operate of Cd tolerance was additional validated by way of heterologous expression, which prompt that SNAC1, RALF12, OsBI-1 can confer Cd tolerance in yeast and tobacco leaves. Concerning the genes concerned in ion transport, the validated Cd-tolerant heavy metal-associated area (HMAD) isoprenylated protein HIPP42 was significantly noteworthy. Additional elucidation of those genes related to Cd tolerance in rice will profit agricultural actions.

Single-Cell Transcriptomics of Immune Cells: Cell Isolation and cDNA Library Technology for scRNA-Seq

Single-cell RNA-sequencing (scRNA-seq) permits a complete evaluation of the transcriptome of particular person cells by next-generation sequencing. ScRNA-seq gives an unbiased method to analyze the mobile heterogeneity and dynamics of numerous organic programs, together with the immune system. Optimization of the technical procedures carried out previous to RNA-seq evaluation is crucial to the success of a scRNA-seq experiment.

Right here, three main experimental procedures are described: (1) the isolation of immune CD8a⁺ T cells from main murine tissue, (2) the technology of single-cell cDNA libraries utilizing the 10× Genomics Chromium Controller and the Chromium Single Cell 3′ Answer, and (3) cDNA library high quality management. On this protocol, CD8a⁺ T cells are remoted from murine spleen tissue, however any cell kind of curiosity will be enriched and used for single-cell cDNA library technology and subsequent RNA-seq experiments.

Differential Gene Expression Responding to Low Phosphate Stress in Leaves and Roots of Maize by cDNA-SRAP

Phosphate (Pi) deficiency in soil can have extreme impacts on the expansion, growth, and manufacturing of maize worldwide. On this examine, a cDNA-sequence-related amplified polymorphism (cDNA-SRAP) transcript profiling approach was used to guage the gene expression in leaves and roots of maize underneath Pi stress for seven days. A complete of 2494 differentially expressed fragments (DEFs) have been recognized in response to Pi hunger with 1202 and 1292 DEFs in leaves and roots, respectively, utilizing a complete of 60 primer pairs within the cDNA-SRAP evaluation.

These DEFs have been categorized into 13 differential gene expression patterns. Outcomes of sequencing and purposeful evaluation confirmed that 63 DEFs (33 in leaves and 30 in roots) have been annotated to a complete of 54 genes concerned in numerous teams of organic pathways, together with metabolism, photosynthesis, sign transduction, transcription, transport, mobile processes, genetic info, and organismal system.

This examine demonstrated that (1) the cDNA-SRAP transcriptomic profiling approach is a robust methodology to investigate differential gene expression in maize exhibiting advantageous options amongst a number of transcriptomic strategies; (2) maize undergoes a fancy adaptive course of in response to low Pi stress; and (3) a complete of seven differentially expressed genes have been recognized in response to low Pi stress in leaves or roots of maize and may very well be used within the genetic modification of maize.

Identification of mobile inhibitors towards Chikungunya virus replication by a cDNA expression cloning mixed with MinION sequencing

cDNA expression cloning has been proven to be a robust method within the seek for mobile elements that management virus replication. On this examine, cDNA library screening utilizing a pool of cDNA derived from interferon-treated human cells was mixed with the MinION sequencer to determine mobile genes inhibiting Chikungunya virus (CHIKV) replication. Problem an infection of CHIKV to Vero cells transduced with the cDNA library produced virus-resistant cells.

Then, the MinION sequence of cDNAs extracted from the surviving cells revealed that the open studying frames of TOM7, S100A16, N-terminally truncated type of ECI1 (ECI1ΔN59), and RPL29 have been inserted in most of the cells. Importantly, the transient expression of TOM7, S100A16, and ECI1ΔN59 was discovered to inhibit the replication of CHIKV in Huh7 cells, indicating that these mobile elements have been doubtlessly anti-CHIKV molecules. Thus, our examine demonstrated that cDNA expression cloning mixed with the MinION sequencer allowed a fast and complete detection of mobile inhibitors towards CHIKV.

Dissecting effectivity of a 5′ fast amplification of cDNA ends (5′-RACE) method for profiling T-cell receptor beta repertoire

Deep sequencing of T-cell receptor (TCR) genes is highly effective at profiling immune repertoire. To arrange a TCR sequencing library, multiplex polymerase chain response (mPCR) is extensively utilized and is very environment friendly. That’s, most mPCR merchandise comprise the area crucial for antigen recognition, which additionally signifies common V(D)J recombination.

Multiplex PCR, nevertheless, could endure from primer bias. A promising different is 5′-RACE, which avoids primer bias by making use of just one primer pair. In 5′-RACE information, nevertheless, non-regular V(D)J recombination (e.g., TCR sequences with out a V gene phase) has been noticed and the frequency varies (30-80%) between research. This means that the reason for or how you can cut back non-regular TCR sequences is just not but well-known by the science group. Though it’s doable to take a position the trigger by evaluating the 5′-RACE protocols, cautious experimental affirmation is required and such a scientific examine continues to be not obtainable.

Right here, we examined the 5′-RACE protocol of a industrial equipment and demonstrated how a modification elevated the fraction of normal TCR-β sequences to >85%. We additionally discovered a robust linear correlation between the fraction of brief DNA fragments and the proportion of non-regular TCR-β sequences, indicating that the presence of brief DNA fragments within the library was the primary reason behind non-regular TCR-β sequences.

Parotid secretory protein Antibody
abx431531-200ul	Abbexa	200 ul	EUR 343.2

Parotid Membrane Tumor Lysate
XBL-10807	ProSci	0.1 mg	EUR 1023
Description: Human parotid tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human parotid tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated parotid tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated parotid tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Parotid Dissociation System 3 (Acinar, Exorbital Iacrimal, parotid), Mouse and Rat
4-20393	CHI Scientific	ea	Ask for price

Bovine SALIVARY PAROTID 25 EA*
57142-2	Pel-Freez	25 EA	EUR 493

Rabbit SALIVARY PAROTID Y 25 EA*
41241-2	Pel-Freez	25 EA	EUR 382.07

Rabbit SALIVARY PAROTID MATR 25 EA*
41342-2	Pel-Freez	25 EA	EUR 382.07

Frozen Tissue Section - Human Tumor: Parotid
T1235190	Biochain	5 slides	EUR 405.6
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Anti-Parotid secretory protein (mouse) antibody
STJ72465	St John's Laboratory	100 µg	EUR 312

cDNA Synthesis SuperMix
20-abx09801420ulSystems	Abbexa	EUR 678.00 EUR 577.20	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

Novo? cDNA Kit
M1165-100	Biovision		EUR 424.8

Novo? cDNA Kit
M1165-25	Biovision		EUR 320.4

Evo? cDNA Supermix
M1168-100	Biovision		EUR 457.2

Evo? cDNA Supermix
M1168-25	Biovision		EUR 320.4

Novo? cDNA Supermix
M1169-100	Biovision		EUR 529.2

Novo? cDNA Supermix
M1169-25	Biovision		EUR 346.8

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis
C1634310	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn
C1634330	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange
C1634340	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato
C1634350	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice
C1634360	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat
C1634390	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human anti-parotid duct antibody ELISA Kit
201-12-0451	SunredBio	96 tests	EUR 528
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Rabbit anti parotid duct antibody ELISA kit
E04A2104-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rabbit anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit anti parotid duct antibody ELISA kit
E04A2104-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Rabbit anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit anti parotid duct antibody ELISA kit
E04A2104-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Rabbit anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Total Protein from Human Tumor Tissue: Parotid
P1235190	Biochain	1 mg	EUR 553.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Pig anti parotid duct antibody ELISA kit
E07A2104-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig anti parotid duct antibody ELISA kit
E07A2104-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig anti parotid duct antibody ELISA kit
E07A2104-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Porcine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human anti parotid duct antibody ELISA kit
E01A2104-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human anti parotid duct antibody ELISA kit
E01A2104-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human anti parotid duct antibody ELISA kit
E01A2104-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Human anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat anti parotid duct antibody ELISA kit
E06A2104-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat anti parotid duct antibody ELISA kit
E06A2104-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat anti parotid duct antibody ELISA kit
E06A2104-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Goat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse anti parotid duct antibody ELISA kit
E03A2104-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse anti parotid duct antibody ELISA kit
E03A2104-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse anti parotid duct antibody ELISA kit
E03A2104-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Mouse anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog anti parotid duct antibody ELISA kit
E08A2104-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog anti parotid duct antibody ELISA kit
E08A2104-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog anti parotid duct antibody ELISA kit
E08A2104-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Canine anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat anti parotid duct antibody ELISA kit
E02A2104-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat anti parotid duct antibody ELISA kit
E02A2104-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat anti parotid duct antibody ELISA kit
E02A2104-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Rat anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human anti-parotid duct antibody ELISA Kit
GA-E0467HM-48T	GenAsia Biotech	48T	EUR 346.8

Human anti-parotid duct antibody ELISA Kit
GA-E0467HM-96T	GenAsia Biotech	96T	EUR 559.2

Monkey anti parotid duct antibody ELISA kit
E09A2104-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey anti parotid duct antibody ELISA kit
E09A2104-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey anti parotid duct antibody ELISA kit
E09A2104-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Monkey anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Parotid secretory protein, Psp ELISA KIT
ELI-16790m	Lifescience Market	96 Tests	EUR 1038

Membrane Protein from Human Tumor Tissue: Parotid
P3235190	Biochain	0.1 mg	EUR 759.6
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Paraffin Tissue Section - Human Adult Normal: Parotid
T2234190	Biochain	5 slides	EUR 253.2
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Frozen Tissue Section - Human Adult Normal: Parotid
T1234190	Biochain	5 slides	EUR 418.8
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Total RNA from Human Tumor Tissue: Parotid
R1235190-10	Biochain	10 ug	EUR 421.2
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human anti-parotid duct antibody ELISA Kit
QY-E02562	Qayee Biotechnology	96T	EUR 433.2

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)
20-abx09801620ulSystems	Abbexa	EUR 744.00 EUR 627.60	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)
20-abx09802120ulSystems	Abbexa	EUR 1045.20 EUR 794.40	100 rxns × 20 ul Systems 50 rxns × 20 ul Systems

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean
C1634370	Biochain	40 reactions	EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Tetro cDNA Synthesis Kit
BIO-65042	Bioline	30 Reactions	Ask for price

Tetro cDNA Synthesis Kit
BIO-65043	Bioline	100 Reactions	Ask for price

SensiFAST cDNA Synthesis Kit
BIO-65053	Bioline	50 Reactions	Ask for price

SensiFAST cDNA Synthesis Kit
BIO-65053/S	Bioline	Sample	Ask for price

SensiFAST cDNA Synthesis Kit
BIO-65054	Bioline	250 Reactions	Ask for price

cDNA Probe Diluent Solution
AR0063	BosterBio	5mL	EUR 127.2

cDNA from Arteriosclerosis: Aorta
C1236012Hd-4	Biochain	40 reactions	EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Artery
C1236013Hd-2	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Arteriosclerosis: Artery
C1236013Hd-4	Biochain	40 reactions	EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Vein
C1236020Hd-2	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Colon
C1236090Lup	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Heart
C1236122Hd-2	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Heart
C1236122Lup	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Kidney
C1236142Hd-2	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Kidney
C1236142Lup	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Liver
C1236149Lup	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Asthma: Lung
C1236152Ld-1	Biochain	40 reactions	EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Bronchitis: Lung
C1236152Ld-2	Biochain	40 reactions	EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Emphysema: Lung
C1236152Ld-3	Biochain	40 reactions	EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pneumonia: Lung
C1236152Ld-4	Biochain	40 reactions	EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Lung
C1236152Lup	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Pancreas
C1236188Lup	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spleen
C1236246Lup	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: stomach
C1236248Lup	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human eNOS cDNA probe
eNOS51-D-2	Alpha Diagnostics	2 ug	EUR 534

Evo™ cDNA Kit
M1164-100	Biovision		Ask for price

Evo™ cDNA Kit
M1164-25	Biovision		Ask for price

Novo? Transcriptome cDNA Kit
M1167-100	Biovision		EUR 1142.4

Novo? Transcriptome cDNA Kit
M1167-25	Biovision		EUR 529.2

OneScriptPlus cDNA Synthesis Kit
G235	ABM	25 x 20 ul reactions	EUR 116.4

OneScriptPlus cDNA Synthesis Kit
G236	ABM	100 x 20 ul reactions	EUR 202.8

OneScriptPlus cDNA Synthesis SuperMix
G453	ABM	25 x 20 ul reactions	EUR 116.4

OneScriptPlus cDNA Synthesis SuperMix
G454	ABM	100 x 20 ul reactions	EUR 202.8

circRNA cDNA Synthesis Kit
G627	ABM	25 rxn (20 ul/rxn)	EUR 370.8

Plant Tissue cDNA: Arabidopsis
PC34-310	Alpha Diagnostics	10 rxn	EUR 498

Pancreas Dissociation System 5 (Acinar, mouse parotid), Mouse
4-20375	CHI Scientific	ea	Ask for price

Pancreas Dissociation System 6 (Acinar, rat parotid), Rat
4-20376	CHI Scientific	ea	Ask for price

Parotid Dissociation System 1 (Epithelial), Mouse and Rat
4-20391	CHI Scientific	ea	Ask for price

Guinea pig anti parotid duct antibody ELISA kit
E05A2104-192T	BlueGene	192 tests	EUR 1524
Description: A sandwich ELISA for quantitative measurement of Guinea pig anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig anti parotid duct antibody ELISA kit
E05A2104-48	BlueGene	1 plate of 48 wells	EUR 624
Description: A sandwich ELISA for quantitative measurement of Guinea pig anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig anti parotid duct antibody ELISA kit
E05A2104-96	BlueGene	1 plate of 96 wells	EUR 822
Description: A sandwich ELISA for quantitative measurement of Guinea pig anti parotid duct antibody in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Total Protein from Human Adult Normal Tissue: Parotid
P1234190	Biochain	1 mg	EUR 427.2
Description: Can be used for various proteomics studies in both normal and pathological cases. It is an excellent control and suitable for educational purposes. This product is prepared from whole tissue homogenates and has undergone SDS-PAGE quality control analysis. The protein is stored in a buffer with protease inhibitor cocktail fo prevent degradation.

Paraffin Tissue Section - Human Parotid Tumor: Pleomorphic Adenoma
T2235190-5	Biochain	5 slides	EUR 308.4
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Anti-parotid secretory protein (mouse aa146-160) antibody
STJ72466	St John's Laboratory	100 µg	EUR 430.8

Total RNA from Human Adult Normal Tissue: Parotid
R1234190-50	Biochain	50 ug	EUR 213.6
Description: Can be used for various studies in the realm of gene expression and regulation, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA Synthesis SuperMix for qPCR
20-abx09801920ulSystems	Abbexa	EUR 828.00 EUR 678.00	100 rxns ×20 ul Systems 50 rxns × 20 ul Systems

cDNA from Alzheimer's Disease: Brain
C1236035Alz	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Brain
C1236035Lcs	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Parkinson's Disease: Brain
C1236035Par	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dementia: Brain: Hippocampus
C1236052Dem	Biochain	40 reactions	EUR 961.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Depression: Brain: Hippocampus
C1236052Dep	Biochain	40 reactions	EUR 961.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Colon
C1236090Lcs	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Esophagus
C1236106Lcs	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Heart
C1236122Lcs	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Interventricular Septum
C1236130Hd-2	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Kidney
C1236142Lcs	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Liver
C1236149Lcs	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Lung
C1236152Lcs	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pulmonary Embolism: Lung
C1236152Ld-5	Biochain	40 reactions	EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Diaphragm
C1236169Lcs	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Pancreas
C1236188Lcs	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Skin
C1236218Lcs	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Small Intestine
C1236226Lup	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spinal Cord
C1236234Lup	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Spleen
C1236246Lcs	Biochain	40 reactions	EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Monkey (Rhesus) cDNA Tissue: Thyroid
MR34-265	Alpha Diagnostics	10 rxn	EUR 498

Evo? cDNA Kit (gDNA Removal)
M1166-100	Biovision		EUR 457.2

Human Adult cDNA Tissue: Lung
HA-152	Alpha Diagnostics	10 rxn	EUR 498

Human Adult cDNA Tissue: Skin
HA-218	Alpha Diagnostics	10 rxn	EUR 498

Human Adult cDNA Tissue: Testis
HA-260	Alpha Diagnostics	10 rxn	EUR 498

Human Tumor Tissue: Breast cDNA
HT05-090	Alpha Diagnostics	10 rxn	EUR 498

Total-Transcriptome cDNA Synthesis Kit
G904	ABM	25 reactions	EUR 268.8

Total-Transcriptome cDNA Synthesis Kit
G905	ABM	100 reactions	EUR 652.8

Mouse iNOS (macrophage) cDNA probe
iNOS61-D-2	Alpha Diagnostics	2 ug	EUR 534

Accuris qMax cDNA Synthesis Kit
PR2100-C-100	Benchmark Scientific	1 PC	EUR 405.3

Due to this fact, thorough removing of brief DNA fragments from a 5′-RACE library is the important thing to excessive information effectivity. We extremely suggest conducting a fraction size evaluation earlier than sequencing, and the fraction of brief DNA fragments can be utilized to estimate the proportion of non-regular TCR sequences. As deep sequencing of TCR genes continues to be comparatively costly, good high quality management must be priceless.